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Overview
The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit enables you to generate highly reproducible WTA data on the BD Rhapsody™ Single-Cell Analysis System. The kit has been tested and validated to generate consistent, high-quality whole transcriptome data across different workflows and users and a wide range of cell inputs.
Learn more from the BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit Brochure.
FEATURES
The BD Rhapsody™ WTA Amplification Kit allows you to build targeted panels based on your WTA data
The kit:
Allows you to identify the most differentially expressed genes in your samples, which can then be used to construct a targeted panel specific to your research needs
Is designed to work reliably across a wide range of cell inputs from 1,000 to 10,000 cells
Works seamlessly with the BD® Single-Cell Multiplexing Kit and enables you to run multiple samples at once without compromising the quality of your data and to save time and money
PERFORMANCE
The BD Rhapsody™ WTA Amplification Kit generates consistent and reproducible data across multiple users and cell types
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/Batch-Effect-tSNE-Plot-Thumbnail.jpg)
Reproducible data from the same sample processed using two different cartridges
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/WTA-3-User-Sample-Data-Reproducibility-Thumbnail.jpg)
Reproducible WTA data from the same sample processed by three different users
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/WTA-PBMC-Process-Thumbnail-01.jpg)
Consistent data across a wide range of cell inputs
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
The BD Rhapsody™ WTA Amplification Kit has been used to identify major immune cell types
t-SNE plot showing the major immune cell types identified with the BD Rhapsody™ WTA Amplification Kit.
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/WTA-Major-Immune-Cell-Identification-Thumbnail-01.jpg)
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
The BD Rhapsody™ WTA Amplification Kit can be used in combination with the BD® Single-Cell Multiplexing Kit to analyze multiple samples simultaneously
Jurkat and Ramos cells labeled with four unique sample tags from the BD® Human Single-Cell Multiplexing Kit were mixed at an equal ratio (1:1:1:1), pooled and processed using the BD Rhapsody™ Single-Cell Analysis System. Libraries were generated using the BD Rhapsody™ WTA Amplification Kit.
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/WTA-Sample-Processing-Thumbnail-01.jpg)
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
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Brochure
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Protocols and handbooks
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BD Single-Cell Genomics Analysis Setup User Guide
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Single-Cell Analysis Workflow with BD Rhapsody™ Systems
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BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol
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BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol
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BD Single-Cell Genomics Bioinformatics Handbook
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BD Rhapsody™ WTA Amplification Kit Product Insert
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Multiomics protocols (WTA + AbSeq)
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
23-22988-00
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