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Reagents
- Flow Cytometry Reagents
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Western Blotting and Molecular Reagents
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Flow Cytometry Reagents
- Immunoassay Reagents
- Single Cell Multiomics Reagents
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Cell Preparation
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Functional Assays
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Microscopy and Imaging Reagents
- Western Blotting And Molecular Reagents
- Cell Preparation Separation Reagents
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Protocols
- BSB Protocol
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Setting Compensation Multicolor Flow
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Tissues Section Stain
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Immunomicroscopy
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Immunohistochemical
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Immunofluorescence
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Frozen Tissue
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Parafin Sections
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Fix Perm Kits
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Protocol Direct Immunofluorscence Staining
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Uses of Fc Block
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Stain Lyse Wash
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Stain Lyse No Wash
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Mouse Splenocytes
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Mouse Rat Leukocytes
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Isotype Control
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Indirect Staining Mononuclear Cells
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Immunopurification
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Human PBMCs
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Human Whole Blood Samples
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Escapee Phenomenon
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Agarose Conjugates
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Anti Phosphotyrosine Biotin Conjugates
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Soluble Antibodies
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Rabbit Polyclonal Antibodies
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Monocloncal Antibodies
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Horseradish Peroxidase
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Certified Reagents
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Biotinylated Antibodies
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Agarose Conjugates X712261
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Surface Staining
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Platelet Activation
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Intracellular Staining
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Indirect Immunofluorescence
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Mouse Ige
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Cytokine Elisa
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Induction Fas
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Induction Dx2
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Apoptosis By Treatment Staurosporine
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Cell Death
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Apo Brdu
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Apo Direct
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Human Cyclins
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Detection Ki 67
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Brdu Detection
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Targeted mRNA Protocols
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WTA Protocols
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360040667732 Protocols
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360023293831 AbSeq Protocols
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360039007471 VDJ CDR3 Protocols
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Annexin V Staining Protocol
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Western Blotting with Horseradish Peroxidase Conjugates or Alkaline Phosphatase Conjugates
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Tissue Preparation for Surface Antigen Staining
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Overview
BD Immunoassay ELISA Reagents contain antibody pairs that have each been developed to work together to quantitate soluble analytes in serum, plasma and tissue culture supernatants using sandwich ELISA.
The reagents:
- Come in three convenient sandwich ELISA formats and provide flexibility and cost efficiency for the analysis of tissue culture supernatants
- BD OptEIA™ Kits contain all necessary reagents and precoated 96-well plates for analysis
- BD OptEIA™ Sets include sufficient capture/detection reagents and standards for five or twenty 96-well plates
- Are composed of unlabeled capture antibodies, biotinylated detection antibodies and protein standards
- Offer a wide range of specificities and assay design flexibility with individual reagents as well as convenient set and kit formats
- Provide a cost-effective approach with single-vial reagents offering a wide range of specificities along with assay design flexibility to develop your own immunoassays
BD OptEIA™ Kits provide all necessary components for convenient quantitation of soluble proteins in serum, plasma and tissue culture supernatants using assay technology that has been trusted by researchers for almost 50 years
The kits contain pre-coated 96-microwell plates with all necessary reagents to measure analyte protein levels, eliminating the time and resources required to develop and optimize an assay. The BD OptEIA™ ELISA Kit II format was developed for superior accuracy with serum and plasma specimens.
Sensitivity: Detection as low as 1 pg/mL
Precision: Low inter- and intra-assay variation
Ease of use: 3.5-hour incubation time, room temperature, no shaking
Accuracy: Specially formulated diluents for superb serum spike recovery
BD OptEIA™ Kits contain:
- Precoated 96-well plates
- Lyophilized standards
- Detection antibodies
- Streptavidin-horseradish peroxidase
- Standard/sample diluent
- ELISA diluent
- Wash concentrate
- TMB one-step substrate reagent
- Stop solution
BD OptEIA™ Sets utilize optimized detection antibodies to measure cytokine (and other soluble protein) levels in serum, plasma and tissue culture supernatants
The detection antibodies in most sets are specifically fragmented to reduce background and nonspecific binding to autoantibodies and plasma proteins
such as complement components, acute phase proteins and fibronectin.
The sets are packaged for convenience with sufficient reagents for five or twenty 96-well plates. The components are pretitrated and QC-tested.
Detailed instructions for dilution of the reagents are included with each set, virtually eliminating the need for tedious optimization experiments.
BD OptEIA™ Sets contain:
- Reagents for five or twenty ELISA plates
- Capture antibody
- Biotinylated detection antibody
- Streptavidin-horseradish peroxidase
- Recombinant standard
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Frequently Asked Questions
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Product Information Sheets
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Protocols
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Tools
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Blocking the Plate [ QuickTime ] [ Windows Media ]
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Capture Antibody Preparation and Coating [ QuickTime ] [ Windows Media ]
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Getting Started [ QuickTime ] [ Windows Media ]
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Standard Preparation and Handling [ QuickTime ] [ Windows Media ]
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Standard and Sample Addition [ QuickTime ] [ Windows Media ]
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Stopping the Reaction and Reading the Plate [ QuickTime ] [ Windows Media ]
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TMB Substrate Preparation and Addition [ QuickTime ] [ Windows Media ]
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Troubleshooting: High Background [ QuickTime ] [ Windows Media ]
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Troubleshooting: Low/No Signal [ QuickTime ] [ Windows Media ]
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Troubleshooting: Poor Standard Curve [ QuickTime ] [ Windows Media ]
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Troubleshooting: Well-to-Well Precision [ QuickTime ]
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Washing the Plates [ QuickTime ] [ Windows Media ]
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Working Detector Preparation [ QuickTime ] [ Windows Media ]
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Product List
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
QuickTime is a trademark of Apple Inc. Windows Media is a trademark of Microsoft Corporation.
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