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Reagents
- Flow Cytometry Reagents
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Western Blotting and Molecular Reagents
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Flow Cytometry Reagents
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Cell Preparation
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Functional Assays
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Microscopy and Imaging Reagents
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Protocols
- BSB Protocol
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Setting Compensation Multicolor Flow
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Tissues Section Stain
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Immunomicroscopy
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Immunohistochemical
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Immunofluorescence
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Frozen Tissue
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Parafin Sections
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Fix Perm Kits
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Protocol Direct Immunofluorscence Staining
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Uses of Fc Block
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Stain Lyse Wash
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Stain Lyse No Wash
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Mouse Splenocytes
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Mouse Rat Leukocytes
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Isotype Control
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Indirect Staining Mononuclear Cells
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Immunopurification
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Human PBMCs
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Human Whole Blood Samples
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Escapee Phenomenon
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Agarose Conjugates
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Anti Phosphotyrosine Biotin Conjugates
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Soluble Antibodies
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Rabbit Polyclonal Antibodies
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Monocloncal Antibodies
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Horseradish Peroxidase
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Certified Reagents
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Biotinylated Antibodies
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Agarose Conjugates X712261
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Surface Staining
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Platelet Activation
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Intracellular Staining
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Indirect Immunofluorescence
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Mouse Ige
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Cytokine Elisa
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Induction Fas
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Induction Dx2
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Apoptosis By Treatment Staurosporine
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Cell Death
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Apo Brdu
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Apo Direct
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Human Cyclins
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Detection Ki 67
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Brdu Detection
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Targeted mRNA Protocols
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WTA Protocols
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360040667732 Protocols
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360023293831 AbSeq Protocols
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360039007471 VDJ CDR3 Protocols
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Annexin V Staining Protocol
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Western Blotting with Horseradish Peroxidase Conjugates or Alkaline Phosphatase Conjugates
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Tissue Preparation for Surface Antigen Staining
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Protocols
Cell Labeling:
1. Single Cell Labeling with the BD™ Single-Cell Multiplexing Kits:
The BD™ Human Single-Cell Multiplexing Kit (Cat. No. 633781) and the BD™ Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793) utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the BD™ Human Single-Cell Multiplexing Kit, referred to as a Sample Tag, has a unique sample oligo barcode conjugated to a human universal antibody and every Sample Tag in the BD™ Mouse Immune Single-Cell Multiplexing Kit is conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system.
2. Single Cell Labeling with the BD™ Single-Cell Multiplexing Kit and BD™ AbSeq Ab-Oligos
This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD™ Human Single-Cell Multiplexing Kit (Cat. No. 633781) or BD™ Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793). The BD™ AbSeq Ab-Oligos are used for antigen-expression profiling with BD Rhapsody™ single cell capture and downstream library preparation. Each BD™ AbSeq Ab-Oligo is an oligonucleotide-conjugated antibody that contains an antibody-specific barcode and poly(A)-tail for bead capture, PCR amplification, and library generation. The protocol supports BD™ AbSeq labeling of 20,000 to 1 million cells. Up to 40 antibodies can be pooled together per staining reaction. The BD Single-Cell Multiplexing Kits utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the BD™ Human Single-Cell Multiplexing Kit, referred to as a Sample Tag, has a unique sample oligo barcode conjugated to a human universal antibody and every Sample Tag in the BD™ Mouse Immune Single-Cell Multiplexing Kit is conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system. You can co-label cells with Sample Tags and BD AbSeq Ab-Oligos in a single tube, or you can sequentially label cells with Sample Tags and pool cells before labeling with BD AbSeq Ab-Oligos.
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