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Overview
The lyophilized panels reduce inter-experiment variability and come with long shelf lives making them ideal choices for multisite and longitudinal studies. A wide variety of reagents and dyes are available for cocktailing, including BD Horizon Brilliant™ Dye Reagents. Lyophilized panels can be manufactured in a variety of packaging options to meet customer requirements including tubes, microtubes, plates and multi-test vials.
Performance
This lyophilized cocktail demonstrates similar performance to liquid reagents as shown by MFI for the two reagents we compared.
Additional BD Lyophilized Products
In addition to our lyophilized panels, we also offer BD Lyoplate™ Plates, lyophilized beads and lyophilized cells.
Lyophilized PBMCs as controls
Lyophilized cells are excellent as staining, instrument setup or gating controls. We can manufacture lyophilized PBMCs and cell lines. In this experiment, freshly fixed, permeabilized and stained PBMCs were compared to lyophilized cells that were stained with conjugates to CD69 and IFN-γ (left and middle). Freshly prepared cells were also compared to cells that were stained and then lyophilized (right). There was virtually no difference in signal intensity or cellular events between these three preparations.
Reference
Chan RC, Kotner JS, Chuang CM, Gaur A. Stabilization of pre-optimized multicolor antibody cocktails for flow cytometry applications. Cytometry B Clin Cytom. 2017;92(6):508-524. doi: 10.1002/cyto.b.21371
Class 1 Laser Product. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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