-
Reagents
- Flow Cytometry Reagents
-
Western Blotting and Molecular Reagents
-
Flow Cytometry Reagents
- Immunoassay Reagents
- Single Cell Multiomics Reagents
-
Cell Preparation
-
Functional Assays
-
Microscopy and Imaging Reagents
- Western Blotting And Molecular Reagents
- Cell Preparation Separation Reagents
- Functional Cell Based Reagents
- Microscopy Imaging Reagents
- Single Cell Multiomics Reagents
- Single Cell Multinomics Reagents
-
Protocols
- BSB Protocol
-
Setting Compensation Multicolor Flow
-
Tissues Section Stain
-
Immunomicroscopy
-
Immunohistochemical
-
Immunofluorescence
-
Frozen Tissue
-
Parafin Sections
-
Fix Perm Kits
-
Protocol Direct Immunofluorscence Staining
-
Uses of Fc Block
-
Stain Lyse Wash
-
Stain Lyse No Wash
-
Mouse Splenocytes
-
Mouse Rat Leukocytes
-
Isotype Control
-
Indirect Staining Mononuclear Cells
-
Immunopurification
-
Human PBMCs
-
Human Whole Blood Samples
-
Escapee Phenomenon
-
Agarose Conjugates
-
Anti Phosphotyrosine Biotin Conjugates
-
Soluble Antibodies
-
Rabbit Polyclonal Antibodies
-
Monocloncal Antibodies
-
Horseradish Peroxidase
-
Certified Reagents
-
Biotinylated Antibodies
-
Agarose Conjugates X712261
-
Surface Staining
-
Platelet Activation
-
Intracellular Staining
-
Indirect Immunofluorescence
-
Mouse Ige
-
Cytokine Elisa
-
Induction Fas
-
Induction Dx2
-
Apoptosis By Treatment Staurosporine
-
Cell Death
-
Apo Brdu
-
Apo Direct
-
Human Cyclins
-
Detection Ki 67
-
Brdu Detection
-
Targeted mRNA Protocols
-
WTA Protocols
-
360040667732 Protocols
-
360023293831 AbSeq Protocols
-
360039007471 VDJ CDR3 Protocols
-
Annexin V Staining Protocol
-
Western Blotting with Horseradish Peroxidase Conjugates or Alkaline Phosphatase Conjugates
-
Tissue Preparation for Surface Antigen Staining
-
Account Support
-
Account FAQs
- Account FAQ Answer 1
- Account FAQ Answer 2
- Account FAQ Answer 3
- Account FAQ Answer 4
- Account FAQ Answer 5
- Account FAQ Answer 6
- Account FAQ Answer 7
- Account FAQ Answer 8
- Account FAQ Answer 9
- Account FAQ Answer 10
- Account FAQ Answer 11
- Account FAQ Answer 12
- Account FAQ Answer 13
- Account FAQ Answer 14
- Account FAQ Answer 15
- Account FAQ Answer 16
- Account FAQ Answer 21
- Create Account
- Manage Account Settings
-
PrivacyPolicy
-
Terms and Conditions
-
Account FAQs
-
- Account FAQ Answer 1
- Account FAQ Answer 2
- Account FAQ Answer 3
- Account FAQ Answer 4
- Account FAQ Answer 5
- Account FAQ Answer 6
- Account FAQ Answer 7
- Account FAQ Answer 8
- Account FAQ Answer 9
- Account FAQ Answer 10
- Account FAQ Answer 11
- Account FAQ Answer 12
- Account FAQ Answer 13
- Account FAQ Answer 14
- Account FAQ Answer 15
- Account FAQ Answer 16
- Account FAQ Answer 21
- Korea (English)
- Korea (Korea)
- 국가 / 언어 변경
Old Browser
Flow cytometric analysis of Stat1 (pY701). U-937 cells (Human histiocytic lymphoma; ATCC CRL-1593.2) were either left unstimulated (unshaded) or stimulated (shaded) with 1000 U/mL recombinant human IFN-γ (Cat. No. 554617) for 15 minutes at 37°C. Cells were fixed with BD Cytofix™ buffer (Cat. No. 554655) for 10 minutes at 37°C and then permeabilized by adding BD Phosflow™ Perm Buffer III (Cat. No. 558050 for 30 minutes on ice. Cells were then washed twice in BD Pharmingen™ Stain Buffer (Cat. No. 554656) and stained with the Alexa Fluor® 488 mouse anti-Stat1 (pY701) antibody. Cells were analyzed on a BD FACSCalibur™ flow cytometery instrument. For intracellular staining of human whole blood, BD Phosflow™ Lyse/Fix buffer (Cat. No. 558049) may be used for fixation.
BD™ Phosflow Alexa Fluor® 488 Mouse Anti-Stat1 (pY701)
규제 상태 범례
Becton, Dickinson and Company의 명시적인 서면 승인 없이는 사용 하실 수 없습니다.
준비 및 보관
권장 분석 절차
For more information about BD Phosflow™ products and protocols, investigators are encouraged to visit http://www.bdbiosciences.com/research/ics/resources/index.jsp
제품 고시
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
관련 제품
Stat (Signal transducer and activators of transcription) proteins mediate the biological activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction activates constitutively-associated JAK family kinases as well as subsequent recruitment and activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, the primary transcription activator induced by interferon binding to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1α and 84-kDa Stat1β; Stat1α has 38 additional C-terminal amino acids. In response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex translocates to the nucleus. This forms an active complex that includes the DNA-binding p48 subunit, and is responsible for modulating interferon-stimulated genes (ISGs) transciption.
The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1α and Stat1β.
개발 참고 자료 (6)
-
Bromberg J, Darnell JE. The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000; 19(21):2468-2473. (Biology). 참조 보기
-
Darnell JE Jr. STATs and gene regulation. Science. 1997; 277(5332):1630-1635. (Biology). 참조 보기
-
Fu XY, Zhang JJ. Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter. Cell. 1993; 74(6):1135-1145. (Biology). 참조 보기
-
Perez OD, Mitchell D, Campos R, Gao GJ, Li L, Nolan GP. Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry. Curr Protoc Cytom. 2005; 6.20.1-6.20.22. (Clone-specific: Flow cytometry). 참조 보기
-
Suni MA, Maino VC. Flow cytometric analysis of cell signaling proteins. Methods Mol Biol. 2011; 717:155-169. (Clone-specific). 참조 보기
-
Tanaka S, Saito Y, Kunisawa J, et al. Development of mature and functional human myeloid subsets in hematopoietic stem cell-engrafted NOD/SCID/IL2rgammaKO mice. J Immunol. 2012; 188(12):6145-6155. (Clone-specific: Flow cytometry). 참조 보기
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
23-22944-00
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.