PerCP-Cy™5.5 Mouse Anti-Human CD4

BD™ PerCP-Cy™5.5 Mouse Anti-Human CD4

Name: PerCP-Cy™5.5 Mouse Anti-Human CD4
Brand: BD™
SKU: 341654

Clone SK3 (also known as Leu3a)

(RUO (GMP))

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제품 세부 정보

Brand: BD™

Product Name: CD4 PerCP-Cy5.5 SK3 RUO/GMP

Alternative Name: L3T4; Leu3a; T-cell surface antigen T4/Leu-3 ; W3/25 ; CD4 antigen (p55)

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Peripheral Blood T Cells

Application: Flow cytometry

Concentration: 1.5 μg/mL

Vol. Per Test: 20 μL

Workshop Number: I T38; III T140,496

Entrez Gene ID: 920

RRID: AB_400452

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

준비 및 보관

The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

341654 Rev. 2

항체 세부 정보

SK3

The SK3 monoclonal antibody specifically binds to CD4. The CD4 antigen is a 55 kDa type 1 transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed on T-helper/inducer lymphocytes and monocytes. CD3+CD4+ T cells comprise approximately 28% to 58% of normal peripheral blood lymphocytes. CD4 is also expressed on 80% to 95% of normal thymocytes. The CD4 antigen is present in low density on the cell surface of monocytes and in the cytoplasm of monocytes and macrophages (CD3-CD4+). CD4 serves as a T cell coreceptor for MHC class II antigen recognition and as a receptor for the human immunodeficiency virus (HIV). The SK3 antibody inhibits HIV binding to CD4+ cells.

341654 Rev. 2

형광 세부 정보

PerCP-Cy5.5

BD™ PerCP-Cy5.5 is part of the BD Blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. BD PerCP-Cy5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (eg, a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configuration (lasers and optical filters) are appropriate for this dye. Visit our Supplies, Parts and Setup Reagents page, find the link to your instrument and on the product page, navigate down to Related Products for more information.

형광: PerCP-Cy5.5

Excitation Source: Blue 488 nm

Excitation Max: 482 nm

Emission Max: 676 nm

341654 Rev.2

인용 및 참고 문헌

View product citations for antibody "341654" on CiteAb

개발 참고 자료 (28)

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Barber EK, Dasgupta JD, Schlossman SF, Trevillyan JM, Rudd CE. The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex. Proc Natl Acad Sci USA. 1989; 86:3277-3281. (Biology: Flow cytometry).
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Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology: Flow cytometry). 참조 보기
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모두 보기 (28)

341654 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not For Use in Diagnostic Procedures.

RUO/GMP—For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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