BD FACSCelesta™ Cell Analyzer

The cell analyzer designed to make multicolor flow cytometry more accessible with the ability to detect and analyze up to 14 parameters in a single sample

Overview

Choose from four instrument configurations. All configurations have blue (488-nm) and violet (405-nm) lasers, which can be paired with a red (640-nm), ultraviolet (355-nm) or yellow-green (561-nm) laser, depending on your application needs.

Watch the video describing the ease of use of the BD FACSCelesta™ Flow Cytometer.

BD FACSCelesta Multicolor Flow Cytometer: Ease of Use Overview

FEATURES

The BD FACSCelesta™ Cell Analyzer enables deep and powerful insights in cell analysis at your benchtop

4 different laser configurations within a compact footprint
Fluidics capable of acquiring tube or plate formats
Known and trusted B D FACSDiva™ Software helps streamline flow cytometry workflows

Get more information from the B D FACSCelesta™ Cell Analyzer Brochure.

Explore the configuration sheets for different laser combinations:

BD FACSCelesta™ Flow Cytometer Configuration Sheet – BV

BD FACSCelesta™ Flow Cytometer Configuration Sheet – BVR

BD FACSCelesta™ Flow Cytometer Configuration Sheet – BVUV

BD FACSCelesta™ Flow Cytometer Configuration Sheet – BVYG

The BD FACSCelesta™ Cell Analyzer is optimized for use with BD Horizon Brilliant™ Dyes

More parameters mean more impact

The optical and electronics system—lasers, filters, detectors, optical paths and signal processing technologies—have been engineered for use with BD Horizon Brilliant™ Dyes.
A system of dichroic mirrors and bandpass filters, packed into a compact polygon, splits off light from each laser and directs it to the correct detectors
The detectors (photomultiplier tubes or PMTs) themselves fit compactly behind the filter assemblies

Options to enhance and tailor your BD FACSCelesta™ Cell Analyzer toward your specific research needs

BD FACSFlow™ Supply System (FFSS) and BD™ High Throughput Sampler (HTS) options available when you need

The optional FFSS fluidics cart increases capacity and ease of use while maintaining a stable fluidics pressure and reduces daily maintenance by incorporating two 20-L containers (Cubitainers™)
Fluidic sensors maintain constant pressure, and a fluidics monitoring system warns when sheath fluid is low or empty or when the waste container is full
A digital control panel makes it easy to monitor the operational status of the cytometer and make adjustments
The HTS option provides rapid, fully automated sample acquisition from microtiter plates and supports a wide range of research applications and is compatible with 96-well U, V and flat-bottom plates as well as 384-well microtiter plates
In high-throughput mode, the HTS can process a 96-well plate in less than 15 minutes with less than 1% carryover and standard throughput mode can be selected to acquire larger sample volumes or where longer acquisition times are required

APPLICATIONS

Combining the BD FACSCelesta™ Cell Analyzer and BD Horizon Brilliant™ Polymer Conjugates allows resolution of rare and low-density antigens

Rare cells, or cells that have few surface receptors of a marker of interest, can be difficult to detect using conventional reagents. Bright reagents are essential in resolving these dim cells from others in a sample.

BD Horizon Brilliant™ Dyes can endow previously dim cells with much brighter fluorescence signals than traditional organic fluorescent dyes or even phycobiliproteins such as PE or APC as demonstrated in the figures below.

Optimization for these bright dyes enables the BD FACSCelesta™ System to identify cell populations with a broader range of receptor density.

Explore the data book featuring data collected for various immunology applications using the BD FACSCelesta™ System.

With three lasers and up to 14 optical channels, the BD FACSCelesta™ Flow Cytometer can multiplex many assays at once on the same sample.

Using a broad landscape of cell function assays, flow cytometry can shed light on a variety of sample types, such as whole blood, cell lines and yeast.

Detect and analyze light scatter to measure physical characteristics of cells in suspension, such as cell shape, size and internal complexity

Add fluorescent markers to interrogate expressed or secreted proteins that reveal cell phenotype, function and status