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Overview
The BD FACSymphony™ A5 Cell Analyzer:
- Leverages the inherent benefits of flow cytometry and enables the simultaneous detection of up to 50 parameters
- Offers highly customizable options to meet your research priorities and can be configured to meet your needs today with room for growth tomorrow
- Harnesses the power of the full BD solution to reach deeper insights with state-of-the-art analyzers, a portfolio of bright dyes, specialized technical support and high-dimensional analysis tools
Contact your local representative to discuss the ideal instrument to meet your needs.
Learn more from the BD FACSymphony™ A5 Cell Analyzer brochure.
Find out more about the BD FACSymphony™ A3 Cell Analyzer.
FEATURES
The BD FACSymphony™ A5 Cell Analyzer improves sensitivity to enable you to identify and analyze rare cell types and events
The instrument features an ultra-quiet VPX electronics system that supports up to 50 high-performance detectors.
The capabilities of this platform technology uniquely allow you to conduct deep and broad phenotyping and gain richer scientific insights by fully leveraging the broad portfolio of catalog and custom BD Horizon Brilliant™ Reagents.
As a Special Order Research Product (SORP), the BD FACSymphony™ A5 Cell Analyzer offers a choice of lasers from 26 different laser wavelengths to optimally configure your instrument for your specific research application.
For most lasers, multiple power ratings that can be adjusted, stored and recalled using the digital laser command and control functionality are available.
Dependent on the laser, power settings from 20–1,000 mW may be available.
Fluorochrome availability and excitation characteristics across various wavelengths should be discussed during the configuration process to identify the best optical options for your research.
The BD FACSymphony™ A5 Cell Analyzer allows you to select up to 9 lasers for maximum flexibility with new lasers, dyes and applications in the pipeline
Configure your instrument to meet your research needs using traditional or unique wavelengths and power ratings.
Optical arrays offer more flexibility than ever
Choose from:
- Classic tube photomultiplier tubes (PMTs) in decagon arrays with up to 10 detectors
- Small square form PMT and GaAs/GaAsP* detectors in high-parameter cascade (HPC) arrays
- Detectors optimized for increased sensitivity and lower noise in ranges of the spectrum
- Up to 20 detectors per laser
- Discrete pre-amplifiers
Filter sets are exchangeable for traditional fluorescent markers as well as specialized sets for unique dyes or proteins.
* Gallium arsenide phosphide
The BD® High Throughput Sampler (HTS) and BD FACSFlow™ Supply System (FFSS) options are available for increased efficiency
The HTS option:
- Automates and accelerates sample acquisition
- Compatible with 96- and 384-well plates
- <0.5% sample carryover in high-throughput mode
The FFSS option:
- Increases capacity and ease of use while maintaining a stable fluidics pressure
- Reduces daily maintenance by incorporating a 20-L BD FACSFlow™ Cubitainer
Common lasers used:
Laser | Wavelength (nm) | Power (mW) |
UV | 355 | 20–100 |
Violet | 405 | 100–200 |
Blue | 488 | 100–200 |
Yellow Green | 561 | 100–200 |
Red | 637 | 140 |
PERFORMANCE
The BD FACSymphony™ A3 Cell Analyzer works with conventional and BD Horizon Brilliant™ Fluorochromes to expand experimental design and help accelerate time to insight.
Enable on-site training and instrument characterization with CD4 fluorochrome evaluation kits
Reduce cost and risk of optimizing new high-parameter panels
The BD FACSymphony™ A3 Cell Analyzer and FlowJo™ v10 Software enable high-dimensional data analysis
Take your analysis to the next level with new features in FlowJo™ v10 Software including improved BD FACSDiva™ Software support, platform overlays, spectral compensation and more.
Useful tools for high-dimensional visualization and analysis of BD FACSymphony™ Cell Analyzer data include t-SNE, OptSNE, UMAP, TriMAP and EmbedSOM.
Keep up to date on the latest plugins at FlowJo Exchange.
APPLICATIONS
- Brochures
- Data Sheet
- Job Aid
- Technical Specifications and Guides
- Configuration Guide
Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
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