BD Accuri™ C6 Plus Personal Flow Cytometer

The cell analyzer with capabilities to bring flow cytometry even more within reach for new and experienced researchers alike.

Overview

The BD Accuri™ C6 Plus Flow Cytometer, with its compact 11 x 14.75 x 16.5-inch footprint, light weight of 30 lb and operational simplicity, supports a wide variety of applications including immunology, cell and cancer biology, plant and microbiology and industrial applications. This portable and compact cell analyzer holds the analytical power of a cytometer twice its size. Backed by 23,000 citations to date*, the BD Accuri™ C6 Plus System enables you to go beyond your current research potential.

Get information and resources for the discontinued BD Accuri™ C6 Personal Flow Cytometer .

*Google Scholar™: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Accuri&btnG=

Features

The BD Accuri™ C6 Plus Flow Cytometer Advantages

Sensitive, reliable and capable

Reliable instrument performance with automated QC
Volumetric counting and continuous sampling

Simple: easy to learn, use, maintain and teach

Quick and easy instrument setup
Simplified workflow with BD quality reagents and kits
Walkaway convenience with automated sample loader

Compact and transportable

Small footprint for easy transportation between labs

Intuitive and easy-to-learn BD Accuri™ C6 Plus Software

Intuitive user interface
Easy to learn and data can be collected and analyzed in less than an hour
Clearly visible software options and instrument controls

Get more information from the BD Accuri™ C6 Plus System Brochure.

The BD Accuri™ C6 Plus System Accurately Monitors the Sample Volume Pulled Per Run

Fluidics

A unique, nonpressurized, peristaltic pump system drives the fluidics
The system can calculate absolute counts or sample concentration per µL without the use of counting beads

The optional BD CSampler™ Plus Software accessory offers reliable, easy-to-use automation while adding minimal footprint:

48-well plates
96-well plates
96-deep-well plates
24-tube rack (included) for standard 12 x 75-mm tubes

Get more information about the fluidics from the BD Accuri™ C6 Plus Flow Cytometer brochure.

The BD Accuri™ C6 Plus Flow Cytometer, Combined with BD^® Kits and Templates, Provides Better Insight and Easier Workflow

As easy as cell analysis is going to get

Apply the power of flow cytometry to your research with ready-to-go reagent kits and free software templates specific to your studies.

Preconfigured kits include fluorescent antibodies, buffer systems and other reagents needed for acquisition and analysis.

Kits and templates are available for various applications:

Immunophenotyping, apoptosis, cell cycle, microbial counting, intracellular cytokine studies, stem cell analysis and more.

The BD Accuri™ C6 Plus Cell Analyzer Provides Simplicity and Sensitivity on Your Benchtop

Optics

Equipped with a blue and red laser, two light scatter detectors and four fluorescence detectors with optical filters optimized for the detection of many popular fluorochromes, including FITC, PE, PerCP-Cy™5.5 and APC
Easy to use with no need to adjust detector voltage
Data are digitally collected over a wide dynamic range and are fully available to you as needed, eliminating the risk of data loss due to incorrect settings
Software functions such as Zoom and VirtualGain allow visualization of data at any scale, so you can precisely set gates and regions
Extraordinary fluorescence sensitivity with optical and fluidic design provide the ability to withstand rugged conditions. Provided the system is anchored, it can run samples even if the benchtop is in motion, such as aboard a ship

Find out about BD Accuri™ C6 Plus System fluorochrome support.

Excitation Wavelength	Color	Fluorochromes/Dyes	Standard Filter	Optional Filter
488 nm	TBD	FITC, BB515, Alexa Fluor™ 488, CFSE Hex: 41ad49	533/30	-
	TBD	GFP Hex: 41ad49	533/30	510/15
	TBD	JC-1, Fluo-4 AM, SYBR™Green Hex: 41ad49	533/30	-
	TBD	YFP Hex: fddb03	533/30	540/20
	TBD	PE, JC-1, BD™ MitoStatus TMRE Hex: eae553	585/40	-
	TBD	OFP Hex: d9a254	585/40	565/20
	TBD	Propidium Iodide Hex: e48e25	585/40, 670 LP	610/20
	TBD	BD Horizon™ PE-CF594 Hex: e48e25	670 LP	610/20
	TBD	RFP, mCherry, dsRed Hex: db5e56	670 LP	610/20
	TBD	PerCP, PE-Cy5 Hex: db5e56	670 LP	-
	TBD	PerCP-Cy5.5 Hex: db5e56	670 LP	-
	TBD	7-AAD Hex: db5e56	670 LP	780/60
	TBD	PE Cy™7 Hex: 982121	670 LP	780/60
640 nm	TBD	APC, BD™ MitoStatus Red, Alexa Fluor™ 647	675/25 LP	-
640 nm	TBD	APC-H7, APC-Cy7 Hex: 982121	670 LP	780/60

Performance

Adaptable optics, with many filter, laser and configuration options, and the unique fluidics system provide flexibility in the fluorochrome combinations you can analyze and automation for direct counting of cells

Identification of blood cell populations in a single tube using four colors

Bacterial viability on the BD Accuri™ C6 Plus System.

Applications

BD Accuri™ Flow Cytometers Enable Immunology Research and Immunophenotyping of Cell Populations

Several features of BD Accuri™ Flow Cytometers make them ideal platforms for differential analysis of human peripheral blood samples.

Fixed-voltage detectors simplify data collection and reduce the potential for data loss due to signal over- or under-amplification
The broad dynamic range makes it easy to analyze populations as varied in size as platelets and eosinophils in the same data file
The easy-to-use software Zoom tool allows precise control when setting gates.
Direct volume measurement allows direct calculation of the cell concentration per µL for each population of interest

View Technical Bulletin describing the identification of human peripheral blood cell populations with the BD Accuri™ Flow Cytometer.

BD Accuri™ Flow Cytometers Have Been Used in Cell and Cancer Biology Applications

With two lasers and four fluorescence detectors, the BD Accuri™ Personal Flow Cytometers bring versatility and convenience to your benchtop. Two additional features can further simplify your research:

Absolute counts: Cell concentration can be calculated directly from BD Accuri™ Software statistics tables without the addition of counting beads.

Kits and templates: Include needed reagents and buffers to assess apoptosis, cell cycle and proliferation and DNA damage. Free BD Accuri™ Software templates simplify data collection and analysis.

Detecting apoptosis, DNA damage and cell proliferation using the kit and template.

Technical Bulletin: Multiple Methods for Detecting Apoptosis on the BD Accuri Flow Cytometer
Product Information Sheet: BD Apoptosis Kits and Templates
Product Information Sheet: BD Cell Cycle/DNA Kits and Templates

Use of BD Accuri™ Flow Cytometers in Microbiology Applications

Microbe counts and concentrations can be calculated rapidly, directly and automatically from the software statistics tables without the addition of counting beads
If microbes are transfected with green, yellow, red or other fluorescent proteins, they can be detected and clearly differentiated from non-transfected populations
BD Accuri™ Systems are ideal for monitoring drinking water because of their transportability, open fluidics systems and ability to determine sample volume and calculate cell concentrations directly. A free software template for the Eawag protocol simplifies setup and analysis

Technical Bulletin: A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples
Product Information Sheet: BD Cell Viability Kit
Product Information Sheet: BD Accuri™ Eawag Water Quality Template
White Paper: Assessing Water Quality with the BD Accuri™ Flow Cytometer

Industrial Applications of the BD Accuri™ Flow Cytometer

BD Accuri™ Flow Cytometers are compact and rugged and can be transported into labs, processing facilities and even into the field
The open fluidics system allows it to connect easily to bioreactors and fermentation units for automatic sampling and continuous culture monitoring
The light scatter signals can be used to discriminate cells and fluorescence signals to measure cell viability, vitality and other important cell characteristics
The instruments also interface smoothly with automated bioreactor sampling systems so that samples can be taken continuously and analyzed automatically

White Paper: Bioprocess Monitoring with the BD Accuri™ Flow Cytometer
Access other White Papers on Assessing water quality, Continuous measurement of intracellular calcium, Determining cell concentrations by direct volume, Life science applications of personal flow cytometry, Sowing the seeds of knowledge: the integration of practical flow cytometry into undergraduate education, Rapid and accurate analysis of the nuclear DNA contents of flowering plants.