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      BD FACSymphony™ A5 SE Cell Analyzer

      Unlock the full potential of the BD FACSymphony™ platform with the flexibility of spectral unmixing or compensation workflows

       

       

      Overview

      The BD FACSymphony™ A5 SE Cell Analyzer offers the ability to:
       

      • Gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors
      • Unmix spectral data live in BD FACSDiva™ Software while retaining familiar compensation workflow
      • May improve resolution of difficult cells and populations using autofluorescence unmixing
      • Access spectral abilities on your BD FACSymphony™ A5 instrument with an A5 SE upgrade kit
      • Choose between spectral or compensation-based workflows, achieved through careful optical design and added software functionality
         

      Contact your local representative to learn more.

       

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      Features




      The BD FACSymphony™ A5 SE Cell Analyzer offers an optical design to enable both spectral unmixing and compensation workflows

      Algorithmically optimized filter set collects full spectrum of emitted light
       

      • Algorithm trained on available dyes to optimize placement of 48 detectors across five onboard lasers




      Software functionality allows live spectral unmixing or compensation workflows in BD FACSDiva™ Software

      • Using the familiar BD FACSDiva™ Software workflow, assign a dye to a detector  with the choice to analyze using compensation or spectral unmixing live during acquisition
      • Visualize spectral profile with spectral plots
      • Resolve populations with high autofluorescence using autofluorescence unmixing



      Premium BD FACSymphony™ System technology enhances resolution

      High-parameter cascade (HPC) arrays
      • Scalable detector array technology allows for up to 20 detectors on a single laser line
      • Enables square form fit and gallium arsenide phosphide (GaAsP) PMTs for enhanced resolution

      High-sensitivity GaAsP PMTs

      • Reduce noise and increase sensitivity in red and near-infrared emission channels
      • Maximize resolution across visible spectrum by utilizing two or three GaAsP PMTs off each laser line for emission of 720 nm or greater

      Take your analysis to the next level with FlowJo™ v10.8 Software including improved BD FACSDiva™ Software support

      • Autofluorescence unmixing on both BD FACSDiva™ Software or from 
        Autospill plugin on FlowJo Exchange

      • Useful tools for high-dimensional visualization and analysis of BD FACSymphony™ Cell Analyzer data include t-SNE, OptSNE, UMAP, TriMAP and EmbedSOM

      Keep up to date on the latest plugins at FlowJo Exchange.

       

      The BD® High Throughput Sampler (HTS) and BD FACSFlow™ Supply System (FFSS) options are available for increased efficiency

      The HTS option:

      • Automates and accelerates sample acquisition
      • Compatible with 96- and 384-well plates
      • <0.5% sample carryover in high-throughput mode

      The FFSS option:

      • Increases capacity and ease of use while maintaining a stable fluidics pressure
      • Reduces daily maintenance by incorporating a 20-L BD FACSFlow™ Cubitainer

      Performance

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      The BD FACSymphony™ A5 SE Cell Analyzer offers flexibility of spectral unmixing or compensation-based workflows in a single instrument

      performance1

      Autofluorescence unmixing feature resolves populations with high background autofluorescence in BD FACSDiva™ Software

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      Gallium arsenide phosphide (GaAsP) PMTs reduce noise and increase resolution in far red and near-infrared channels

      What is spectral cytometry?

      Spectral flow cytometry represents an alternative measurement strategy to conventional flow cytometry providing advantages in flexibility of fluorescent label inputs without having to change filters, as well as the ability to multiplex more fluorescent labels in one multicolor sample.


      With the BD FACSymphony™ A5 SE Cell Analyzer, you have the power to choose between spectral or compensation-based workflows. This is achieved through careful optical design and added software functionality. 

       

       

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Class 1 Laser Product.

      Note: For specificities not yet available in the catalog or through BD Optibuild™ Custom Reagents, high-parameter users have access to a small-scale custom conjugation program for BD Horizon Brilliant™ Dyes, including early access to the high-parameter dye menu.

      Alexa Fluor is a trademark of Life Technologies Corporation. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.

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