BD OptiBuild™ On-Demand Reagents

BD OptiBuild™ Reagents are made on demand, which means that every reagent vial is equivalent to a new batch.

If you would like more than 50 µg (1 vial) of the same lot, please contact BDB_Custom_Orders@BD.com. The minimum order size is 500 µg for custom BD OptiBuild™ Reagent lot sizes.

Your reagents are made once you place an order and typically ship within 3 business days in the U.S., Canada, China and EU. For other regions, please contact your local BD representative for appropriate timelines. If more than 10 vials of an individual reagent are ordered, it may take longer than 3 business days to fulfill the order.

No. Placing an order instantly activates the on-demand production of your reagent. Therefore, orders cannot be canceled once the production begins.

BD OptiBuild™ Reagents feature a unique portfolio of products to be available on demand. If you need additional support on substitute availability, please contact our Research Applications team.

Data can be found on our sample data section. There is also a small subset of BD OptiBuild™ products that have data available on individual product pages. While we are working on adding more data, if what you are seeking is not available online, please contact our Research Applications team for more information. 

The BD OptiBuild™ Reagent production process underwent stringent testing and validation with thousands of reagents to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. Examples of lot-to-lot consistency can found in the sample data section.

If you encounter any issues with reagent performance, contact our Research Applications team . The team can provide any available data and discuss it with you to help resolve your issue.

We recommend titrating the reagent in the test setting you will be using to determine the optimal staining concentration for your specific model systems. Please contact our Research Applications support team  for additional information or watch the titration video below.

Watch titration video

Yes. Whenever you use two or more BD Horizon Brilliant™ Dyes (multiple BD OptiBuild™ or in combination with other BD Horizon Brilliant™ Reagents) we recommend the use of Brilliant Stain Buffer. 

Existing BD Horizon™ Isotype Controls are ideal for use with BD OptiBuild™ Reagents. The existing isotype controls were evaluated during development and found to perform effectively with all reagents.

Known adverse effects of free dye have been evaluated and extensively tested in BD OptiBuild™ Reagents, including in multicolor experiments, and have not been witnessed to any extent.

BD OptiBuild™ Reagents have not been tested for use in imaging. However, we have performed limited image testing on frozen mouse spleen sections and have demonstrated initial feasibility.

BD OptiBuild™ Reagents do not require any additional considerations that are not already taken into account when determining buffers and protocols for traditional research reagents. Standard staining buffers such as BD Pharmingen™ Stain Buffer BSA or BD Pharmingen™ Stain Buffer FBS are recommended for use with BD OptiBuild™ products. However, when using two or more BD Horizon™ family of reagents in a multicolor experiment, it is recommended to use BD Horizon™ Brilliant Stain Buffers.

Yes, BD OptiBuild™ Reagents are compatible with standard fix/perm buffers  recommended by BD.

In an effort to minimize our environmental footprint, we do not to include a hard copy of the technical data sheet (TDS) in BD OptiBuild™ products. A printable version of the TDS is available for every BD OptiBuild™ Reagent on the Data Sheets section of the product details page.

There are over 800 cell surface markers available in the BD OptiBuild™ family and we continue to add more over time. Should there be interest in a cell surface marker not currently in our catalog, please contact your local BD representative.  

Currently, intracellular markers are not part of the BD OptiBuild™ Reagent offering, however we are continuously evaluating our portfolio. Should you have a specific request of IC markers, please contact your local BD representative.

Please visit our website in the future to keep updated as our catalog grows.

We do not have every antibody-dye combination available in the portfolio of BD OptiBuild™ Reagents. If there is interest in a particular antibody-flurochrome conjugate, please contact our customs group at BDB_Custom_Orders@bd.com, or visit the custom solutions page.