BD Horizon™ BV786 Dye Profile

BD Horizon™ BV786 dye is a tandem fluorochrome of the BD Horizon™ BV421 dye and an acceptor dye with an emission maximum at 786 nm. The BD Horizon™ BV786 dye offers a bright choice for the violet laser typically as bright as PE on the blue laser.

BD Horizon™ BV786 Dye Profile

BD Horizon™ BV786 dye is a tandem fluorochrome of the BD Horizon™ BV421 dye and an acceptor dye with an emission maximum at 786 nm. The BD Horizon™ BV786 dye offers a bright choice for the violet laser typically as bright as PE on the blue laser.

Clinical Brilliance Elevated

Elevating Panel Design Possibilities with ASRs Conjugated to Two Additional BD Horizon Brilliant™ Dyes

BD continues to offer a portfolio of analyte specific reagents (ASRs), including the most number of violet laser dyes that are available conjugated to ASRs. Now available BD Horizon Brilliant Violet™ 711 (BV711) and BD Horizon Brilliant Violet™ 786 (BV786) ASRs further expand your laboratory’s options in multicolor panel design. BD Horizon™ BV711 and BV786 ASRs can be used on flow cytometers equipped with a violet laser (405 nm) and appropriate filters, such as the 12-color BD FACSLyric™ Flow Cytometer.

New BV711 ASRs Request a Sample New BV786 ASRs Request a Poster for Your Lab ASRs Poster

Clinical Brilliance Elevated

Lot-to-Lot Consistency

Illustrative examples from product development are shown in Figures 1, 2 and 3 which presents little variation in results from lot-to-lot of our BD Horizon Brilliant Violet™ ASRs.

Figure 1.

Lot-to-lot consistency for CD3 BV786 ASR development lots 1 (orange) and 2 (blue). Lysed whole blood gated on lymphocytes.

Figure 2.

Lot-to-lot consistency for CD4 BV711 ASR development lots 1 (orange) and 2 (blue). Lysed whole blood gated on lymphocytes.

Figure 3.

Lot-to-lot consistency for CD13 BV711 ASR development lots 1 (orange) and 2 (blue). Unstained sample in black. Lysed whole blood gated on monocytes.

Brightness

The BD Horizon™ BV711 and BV786 ASRs are typically as bright or brighter than our analogous research use only (RUO) reagents, helping you to resolve dim populations.

Illustrative examples from product development are shown in Figures 4, 5 and 6.

Figure 4.

CD38 BV711 ASR (orange) compared to analogous CD38 BV711 RUO reagent (blue) [P/N: 740830]. Unstained sample in black. The CD38 BV711 ASR development lots are on average 45% brighter based upon positive population MFI. Lysed whole blood gated on monocytes.

Figure 5.

CD5 BV786 ASR (orange) compared to analogous CD5 BV786 RUO reagent (blue) [P/N: 742553]. The CD5 BV786 ASR development lots are on average 29% brighter based upon positive population MFI. Lysed whole blood gated on lymphocytes.

Figure 6.

CD16 BV786 ASR (orange) compared to analogous CD16 BV786 RUO reagent (blue) [P/N: 563690]. Unstained sample in black. The CD16 BV786 ASR development lots are on average 11% brighter based upon positive population MFI. Lysed whole blood gated on granulocytes.

Flexibility

With an expanded portfolio of ASRs for violet laser channels, you will now have increased flexibility in multicolor panel design. This allows more options to move panel markers to the violet laser channels freeing up the blue and red laser channels.

Illustrative examples from product development are shown in Figures 7, 8 and 9.

Figure 7.

CD4 BV711 ASR (orange) compared to CD4 PE ASR (blue) and CD4 PerCP-Cy5.5 ASR (pink). CD4 BV711 ASR development lot is equivalent in brightness to the CD19 PE ASR and 90% brighter than the PerCP-Cy5.5 ASR based upon positive population MFI. Clone: SK3. Lysed whole blood gated on lymphocytes.

Figure 8.

CD4 BV786 (orange) compared to CD4 PE ASR (blue) and CD4 PerCP- Cy5.5 ASR (pink). CD4 BV786 ASR development lot is 37% and 94% brighter than CD4 PE ASR and CD4 PerCP- Cy5.5 ASR, respectively, based upon positive population MFI. Clone: SK3. Lysed whole blood gated on lymphocytes.

Figure 9.

CD14 BV711 (orange) compared to CD14 PE (blue) and CD14 FITC (brown). CD14 BV711 ASR development lot is equivalent in brightness to the CD14 PE ASR and 39% brighter than the FITC ASR based on the positive population MFI. Unstained sample in black. Clone: MᶲP9. Lysed whole blood gated on monocytes.

New ASRs

The following BD Horizon Brilliant™ ASRs are now available for consistency, brightness and channel flexibility.

Description	Clone	ASR Cat. No.	ASR with BD Horizon Brilliant Stain Buffer GPR Cat. No.
CD4 BV711	SK3	664519	664539
CD10 BV711	HI10a	664520	664540
CD13 BV711	L138	664521	664541
CD14 BV711	MᶲP9	664522	664542
CD38 BV711	HB7	664523	664543
CD56 BV711	NCAM16.2	664524	664544
CD123 BV711	9F5	664525	664545
Mouse IgG1 BV711	X40	664526	664546
CD3 BV786	SK7	664527	664547
CD4 BV786	SK3	664528	664548
CD5 BV786	L17F12	664529	664549
CD8 BV786	SK1	664530	664550
CD16 BV786	3G8	664531	664551
CD19 BV786	SJ25C1	664532	664552
CD20 BV786	L27	664533	664553
CD23 BV786	EBVCS-5	664534	664554
CD45 BV786	HI30	664535	664555
HLA-DR BV786	G46-6	664536	664556
Mouse IgG1 BV786	X40	664537	664557

Further expanding the ASR portfolio beyond BD Horizon Brilliant™ ASRs, the following ASRs are now also available to increase panel flexibility.

Description	Clone	ASR Cat. No.
CD22 PE-Cy7	S-HCL-1	664538

Analyte Specific Reagent. Analytical and performance characteristics are not established.

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