![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
-
Reagents
- Flow Cytometry Reagents
-
Western Blotting and Molecular Reagents
-
Flow Cytometry Reagents
- Immunoassay Reagents
- Single Cell Multiomics Reagents
-
Cell Preparation
-
Functional Assays
-
Microscopy and Imaging Reagents
- Western Blotting And Molecular Reagents
- Cell Preparation Separation Reagents
- Functional Cell Based Reagents
- Microscopy Imaging Reagents
- Single Cell Multiomics Reagents
- Single Cell Multinomics Reagents
-
Account Support
-
Account FAQs
- Account FAQ Answer 1
- Account FAQ Answer 2
- Account FAQ Answer 3
- Account FAQ Answer 4
- Account FAQ Answer 5
- Account FAQ Answer 6
- Account FAQ Answer 7
- Account FAQ Answer 8
- Account FAQ Answer 9
- Account FAQ Answer 10
- Account FAQ Answer 11
- Account FAQ Answer 12
- Account FAQ Answer 13
- Account FAQ Answer 14
- Account FAQ Answer 15
- Account FAQ Answer 16
- Account FAQ Answer 21
- Create Account
- Manage Account Settings
-
PrivacyPolicy
-
Terms and Conditions
-
Account FAQs
-
- Account FAQ Answer 1
- Account FAQ Answer 2
- Account FAQ Answer 3
- Account FAQ Answer 4
- Account FAQ Answer 5
- Account FAQ Answer 6
- Account FAQ Answer 7
- Account FAQ Answer 8
- Account FAQ Answer 9
- Account FAQ Answer 10
- Account FAQ Answer 11
- Account FAQ Answer 12
- Account FAQ Answer 13
- Account FAQ Answer 14
- Account FAQ Answer 15
- Account FAQ Answer 16
- Account FAQ Answer 21
- Japan (English)
- Japan (Japanese)
-
Change country/language
Old Browser
Overview
The BD® Cytometric Bead Array (CBA) Solutions measure a variety of soluble and intracellular proteins, including cytokines, chemokines, growth factors and phosphorylated signaling proteins using flow cytometry.
The solutions:
- Enable analysis of up to 30 proteins using just 25 to 50 μL of sample
- Are compatible with almost any standard flow cytometer without the need to purchase expensive single-purpose equipment
- Are available in preconfigured kits or in an open configurable format for unique needs
- BD® CBA Kits—preconfigured for simple set up and to minimize the need for optimization of routine panel
- BD® CBA Flex Sets—open and configurable method of detection to build your own multiplexes for protein quantitation as low as 10 pg/mL
- BD® CBA Enhanced Sensitivity Flex Sets—capable of detecting cytokine concentrations as low as 0.274 pg/mL
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/CBA-Assay.png)
Find more details on BD® Cytometric Bead Array Solutions from the brochure.
BD® CBA Kits are preconfigured by functional areas of biology (for example, Th1/Th2 or inflammatory cytokines) and allow simultaneous measurement of up to seven analytes
The kits provide consistent results with routine panels
- Provide preconfigured panels for ease of use and enable multiplex analysis of complex biological samples on a flow cytometer
- Use capture beads that contain unique amounts of a single red dye, whose unique spectral properties enable analysis of samples on single 488-nm laser or dual-laser (488-nm or 532-nm and 633-nm) flow cytometers
- Are ready to use and complete with all of the buffers and reagents necessary to analyze 80 samples
- Offer assays that are stringently developed for ease of use and rapid data analysis and that use antibody pairs chosen for their sensitivity, reproducibility and quality
- Contain antibody pairs that are evaluated for dynamic range, sensitivity and parallel titration curves to native biological samples
- Provide assay diluent and wash buffers that have been formulated to reduce detrimental effects of serum and plasma proteins on assay performance
- Use FCAP Array™ Analysis Software, which gates on each individual bead population and determines the median fluorescence intensity (MFI) for each analyte in the array and generates a standard curve
BD® CBA Flex Sets allow simultaneous measurement of up to 30 analytes on a flow cytometer equipped with either a 488-nm or 532-nm laser, and a 633-nm laser
The flex sets are open and configurable to maximize flexibility and to make it easy to create multiplex assays
The sets:
- Provide specificities for the detection of human, mouse or rat cytokines, chemokines and growth factors; human immunoglobulins; and cell signaling assays for the detection of phosphorylated cell signaling proteins
- Offer assays formulated for mixing into panels of up to 30-plex and are all sold individually to provide a highly flexible system. All products are available off the shelf so custom orders are not required.
- Contain a unique Master Buffer Kit for each product area (Human Soluble Protein, Mouse/Rat Soluble Protein, and Human Immunoglobulin). All assays within each product area have been verified for performance in single-plex and in multiplexed scenarios to ensure consistency between assay types.
- Make it easy to build a multiplex by following five simple steps. The finished assay can be acquired on a variety of dual-laser flow cytometers and analyzed using FCAP Array™ Software or directly with FlowJo™ v10 Software using a downloadable plug-in.
View step-by-step instructions for building a multiplex using BD® CBA Flex Sets.
BD® CBA Enhanced Sensitivity Flex Sets allow you to detect <1 pg/mL of cytokines and get quantitative results in samples that were below the level of detection using standard BD®
The kits offer a reliable, flexible method for the quantitative detection of multiple proteins in a single serum, plasma or tissue culture supernatant sample
The kits:
- Provide the ability to detect protein as low as 0.274 pg/mL, more than a 35-fold improvement compared to most bead assays that can detect down to 5–10 pg/mL
- Include assays for 10 different cytokines involved in inflammation and leucocyte differentiation that can be combined to create a multiplexed panel
- Provide more data in less time from a single sample by allowing you to analyze up to 10 proteins with just 25 to 50 μL of sample. Other methods such as ELISAs require a similar amount of sample to analyze only one protein.
- Are formulated for combination in multiple configurations and sold individually to provide a highly flexible system. A dedicated Master Buffer Kit contains all the buffers needed for the assay.
- Offer assays that have been characterized for performance in single-plex and multiplex scenarios to ensure consistency across assay types. The antibody pair in each assay is evaluated for dynamic range, sensitivity and parallel titration to native biological samples.
- Provide optimized assay protocols to minimize background and maximize signal while using a two-step detection protocol
- Use FCAP Array™ Analysis Software, which gates on each individual bead population and determines the median fluorescence intensity (MFI) for each analyte in the array and generates a standard curve
BD® CBA Functional Beads allow you to conjugate your own antibody for your protein of interest
The beads provide additional options to enhance flexibility
The unconjugated beads:
- Allow conjugation of antibody or protein in less than four hours using common laboratory supplies along with the buffers in the BD® CBA Functional Bead Conjugation Buffer Kit
- Provide an ideal platform for converting existing ELISAs into bead-based immunoassays that can be mixed with our portfolio of BD® CBA Flex Set assays
- Enable the creation of novel multiplex panels with multiple bead positions
- Offer the ability to prepare up to 1,000 tests in a single reaction, ensuring consistency across a large number of tests
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
PERFORMANCE
All BD® CBA Kit formats provide highly sensitive detection of various cytokines.
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/IL-12p70-TFN-Thumbnail.png)
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/IL-10-IL-6-Thumbnail.png)
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/IL-1Beta-IL-8-Thumbnail.png)
Refer to the comparison of BD® CBA Standards to the NIBSC/WHO International Standards to find the NIBSC conversion factor summary.
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/CBA30-Plex-Assay-Thumbnail.png)
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/Human-PBMCs-Thumbnail.png)
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/HEK-293-And-NIH-3T3-Thumbnail.png)
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/sIL-6R-Thumbnail.png)
Refer to the comparison of BD® CBA Standards to the NIBSC/WHO International Standards to find the NIBSC conversion factor summary.
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/IL-2_Thumbnail.png)
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/cba/IL-6-Thumbnail.png)
Refer to the comparison of BD® CBA Standards to the NIBSC/WHO International Standards to find the NIBSC conversion factor summary.
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
-
Brochures
-
User's Guides and Manuals
-
Human Chemokine Kit
-
Human Inflammatory Cytokines Kit
-
Human Th1/Th2 Cytokine Kit
-
Human Th1/Th2 Cytokine Kit II
-
Human Th1/Th2/Th17 Cytokine Kit
-
Mouse Immunoglobulin Isotyping Kit
-
Mouse Inflammation Kit
-
Mouse Th1/Th2 Cytokine Kit
-
Mouse Th1/Th2/Th17 Cytokine Kit
-
Cell Signaling Master Buffer Kit Instruction Manual
-
Human Enhanced Sensitivity Master Buffer Kit
-
Human Soluble Protein Master Buffer Kit
-
Mouse Enhanced Sensitivity Master Buffer Kit
-
Mouse/Rat Soluble Protein Master Buffer Kit
-
Functional Bead Conjugation Buffer Set
-
FCAP Array™ Software v3.0 User's Guide
-
Product Information Sheets
-
BD® Cytometric Bead Array: BD CBA Human and Mouse Th1/Th2/Th17 Kits
-
BD® CBA Reagents: A complete solution with the BD Accuri™ C6 Flow Cytometer
-
Comparison of BD® CBA Standards to the NIBSC/WHO International Standards
-
Tools for Cytokine and Chemokine Quantitation
-
Cell Signaling Flex Set System
-
BD® CBA Enhanced Sensitivity Flex Set System
-
BD® CBA Human Immunoglobulin Flex Set System: Multiplexed, Sensitive Detection of Soluble Immunoglobulins
-
BD® CBA Reagents: A complete solution with the BD Accuri™ C6 Flow Cytometer
-
BD® CBA Soluble Protein Flex Set System: For Multiplex Analysis of Cytokines, Chemokines, and Growth Factors
-
BD Human IL-17A Reagents: A Comprehensive Portfolio for Inflammation and Autoimmunity Research
-
BD Pharmingen™ Mouse IL-17A Antibodies for Flow Cytometry and Multiplexed Bead Assays
-
Comparison of BD® CBA Standards to the NIBSC/WHO International Standards
-
Product List
-
Webinars
-
Frequently Asked Questions
-
Instrument Setup Guides
-
BD Accuri™ C6 Flow Cytometer Instrument Setup Guide
-
BD Accuri™ C6 Flow Cytometer Software Templates
-
BD FACSAria™ III Cell Sorter Instrument Setup Guide
-
BD FACSAria™ III Cell Sorter Software Templates
-
BD FACSCanto™ II Flow Cytometer Instrument Setup Guide
-
BD FACSCanto™ II Flow Cytometer Software Templates
-
BD FACSVerse™ Flow Cytometer Instrument Setup Guide
-
BD FACSVerse™ Flow Cytometer Software Templates
-
BD LSRFortessa™ Flow Cytometer Instrument Setup Guide
-
BD LSRFortessa™ Flow Cytometer Instrument Software Templates
-
Tools
-
BD Accuri™ C5 Flow Cytometer 8 Peak Bead Template
-
BD Accuri™ C6 Flow Cytometer 8 and 6 Peak Bead Template
-
BD® CBA Kit / BD Accuri™ Flow Cytometer Template
-
BD® CBA Flex Set / BD Accuri™ Flow Cytometer Template
-
BD CSampler™ C6 Accessory 8 and 6 Peak Bead Template 24-Tube Rack
-
BD CSampler™ C5 Accessory 8 Peak Template 24-Tube Rack
-
Bead Library – FCAP Array™ v1.0 Software
-
Bead Library – FCAP Array™ v3.0 Software
-
Cell Signaling Flex Set Compatibility
-
Human Ig Flex Set Compatibility
-
Human Soluble Protein Flex Set Compatibility
-
Human Soluble Receptor Flex Set Compatibility
-
Mouse Soluble Protein Flex Set Compatibility
-
Rat Soluble Protein Flex Set Compatibility
-
References
BD® CBA Flex Sets
Enhanced Sensitivity
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
FCAP Array™ is a trademark of Soft Flow Hungary Ltd.
23-23171-00
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.