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BD Pharmingen™ FITC Rat IgG1, κ Isotype Control
クローン R3-34
Regulatory Statusの凡例
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
調製と保管
推奨アッセイ手順
Recommended Assay Procedure:
The FITC-, PE- and APC-conjugated R3-34 immunoglobulins (Cat. No. 554684; 554685; 554686) are suitable rat IgG1 isotype controls for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized mouse and human cells for flow cytometric analysis. Use at comparable concentrations to antibody of interest (e.g., ≤ 0.5 µg antibody/1 million cells (see image, right panel). For specific methodology, see online protocols or chapter 4 on intracellular staining in Techniques for Immune Function Analysis Application Handbook 1st Edition, both of which are located at our website at www.bdbiosciences.com. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
製品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Following immunization of a rat with mouse immunoglobulin (Ig), the Ig from the R3-34 hybridoma was identified as a non-reactive clone. The R3-34 immunoglobulin was selected as an Ig isotype control following screening for low background staining on a variety of mouse and human cells and tissues.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
開発者向け参考資料 (1)
-
Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology: Flow cytometry, Intracellular staining (flow cytometry), Isotype control). 参考文献を見る
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
23-22944-00
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