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Overview

BD® Small Batch Reagents are preformulated, ready-to-use and leverage BD Horizon™ Dri Technology, which reduces variability and eliminates manual pipetting. Our performance-optimized multicolor panels demonstrate consistent use in a variety of clinical research trials and applications. BD® Small Batch Reagents are backed by innovation, based on consensus guidelines, informed by customer voice and collaborations, and supported by high-impact publications.

 

The reagents include BD Horizon™ Dri Panels and Reagents:

 

  • TBNK + CD20 Panel (Features Slide 1)  
  • Monoset panel (Features Slide 2)  
  • Memory T-cell panel (Features Slide 3)  
  • Treg panel (Features Slide 4)  
  • Tumor and tissue dissociation reagent (TTDR) (Features Slide 5)   
 
performance1

The BD Horizon™ Dri TBNK + CD20 Multicolor Panel is optimized for immune monitoring research

The panel was designed for clinical research based on key feedback from Mass General Hospital and Harvard Medical School. The addition of CD20 to standard TBNK analysis expands the utility of a very common test. This tube has been used in research and clinical trials, from monitoring immune responses to CD20 depletion therapies.

 

The panel:

 

  • Characterizes T, B and NK cells
  • Provides absolute counts when paired with BD Trucount™ Tubes
  • Offers standardization with the all-in-one dried format, reducing variability
  • Eliminates manual pipetting, increasing efficiency
  • Is flexible with open channels for liquid drop-ins
performance1

Panel Composition

Format

Specificity

Clone

FITC

CD3

SK7

PE

CD16/56

B73.1/NCAM16.2

PerCP-CyTM5.5

CD45

2D1

PE-CyTM7

CD4

SK3

APC

CD19

SJ25C1

APC-CyTM7

CD8

SK1

V450

CD20

L27

The BD Horizon™ Dri Monoset Panel is optimized for immunotherapy research and clinical research trials

The panel was designed based on published consensus guidelines to identify and characterize classical, intermediate and nonclassical subsets of monocytes in humans. These levels are quantitated both by absolute number and frequency of each subset. This quantitative value-add is key as it enables standardization of your data across multiple studies. Applications for this tube in research and clinical research trials include but are not limited to cardiology, sepsis, atherosclerosis and as predictive biomarkers for immuno-oncology.

 

The panel:

 

  • Provides standardized quantification (via ABC* units) when paired with BD Quantibrite™ Beads
  • Offers an analysis template with gating strategy for BD FACSuite™ Research Software
  • Reduces variability with the all-in-one dried format and offers standardization
  • Eliminates manual pipetting, increasing efficiency
  • Is flexible with open channels for liquid drop-ins

*Antibody bound per cell

performance1

Panel Composition

Format

Specificity

Clone

FITC

CD3

SK7

PE

CD16/56

B73.1/NCAM16.2

PerCP-CyTM5.5

CD45

2D1

PE-CyTM7

CD4

SK3

APC

CD19

SJ25C1

APC-CyTM7

CD8

SK1

V450

CD20

L27

The BD Horizon™ Memory T-Cell Panel is optimized for cell therapy research and immune system research

This 7-color panel is performance-optimized for use in clinical research and cell therapy applications. The tube contains a dried, pre-aliquoted multicolor cocktail comprising markers that are well-established and widely used for the characterization of CD4 and CD8 naïve and memory T-cell subsets. The identification and characterization of these T-cell subsets are critical in a variety of clinical research applications, including immunity studies, vaccine research and various T-cell therapies.

 

The panel:

 

  • Characterizes naïve, effector, central and stem cell memory T-cell subsets
  • Offers an analysis template with gating strategy for BD FACSuite™ Research Software
  • Reduces variability with the all-in-one dried format and is standardized
  • Eliminates manual pipetting, increasing efficiency
performance1

Panel Composition

Format

Specificity

Clone

FITC

CD197

150503

PE-Cy7

CD95

DX2

APC-R700

CD27

M-T271

APC-H7

CD3

SK7

V450

CD4

SK3

V500C

CD8

SK1

BV605

CD45RA

HI100

The BD Horizon™ Treg Panel is optimized for cell therapy research and immune system research

This 7-color panel is performance-optimized for use in clinical research and cell therapy applications. The tube contains a dried, pre-aliquoted multicolor cocktail comprising markers used for the characterization of FoxP3+ naïve, transitional and effector Treg subsets. Applications for this panel in research and clinical research trials include but are not limited to autoimmunity studies, transplant studies, tumor microenvironment studies and infection.

 

The panel:

 

  • Offers an analysis template with gating strategy for BD FACSuite™ Research Software
  • Reduces variability with the all-in-one dried format and is standardized
  • Eliminates manual pipetting, increasing efficiency
  • Is flexible with open channels for liquid drop-ins
performance1
 

Panel Composition

 
Tube 1

Format

Specificity

Clone

BB515

CD25

2A3

PerCP-Cy™5.5

CD4

SK3

PE-Cy™7

CD45RA

L48

APC

CD161

DX12

APC-R700

CD127

HIL-7R-M21

APC-H7

CD3

SK7

 

 

 
Tube 2

Format

Specificity

Clone

PE

FoxP3

236A/E7

The BD Horizon™ Tumor and Tissue Dissociation Reagent (TTDR) is optimized for tumor microenvironment research

 

The BD Horizon™ TTDR Reagent was designed for gentle and effective dissociation of tumors and tissue for single-cell studies. It is optimized to maximize cell yields, while minimizing cell death and epitope/antigen damage.

 

The reagent:

 

  • Maximizes cell yields during dissociation while minimizing cell death, epitope/antigen damage
  • Dissociates a variety of tumor types to enable and empower single-cell studies
  • Maintains the heterogeneity and diversity of your samples
 
performance1

PERFORMANCE

BD Horizon™ Dri Panels can be used to characterize various immune subtypes.

Snowden E, Porter W, Hahn F, et al. Immunophenotyping and transcriptomic outcomes in PDX-derived TNBC tissue. Mol Cancer Res. 2017;15(4):429-438. doi: 10.1158/1541-7786.MCR-16-0286-T

Sen M, Hahn F, Black TA, et al. Flow based single cell analysis of the immune landscape distinguishes Barrett's esophagus from adjacent normal tissue. Oncotarget. 2019;10(38):3592-3604. doi: 10.18632/oncotarget.26911

Isolation of tumor infiltrating lymphocytes (TILs) using TTDR

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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