Skip to main content Skip to navigation

Overview

The BD OneFlow™ PCD Tube is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.1

 

The BD OneFlow™ PCD Tube, when run in parallel with the BD OneFlow™ PCST, is intended for flow cytometric immunophenotyping of normal and aberrant plasma cells in bone marrow as an aid in the diagnosis of multiple myeloma or other plasma cell disorders.

 

Other reagents belonging to the BD OneFlow™ Solution are  BD OneFlow™ LST, BD OneFlow™ B-CLPD T1BD OneFlow™ PCST,  and BD OneFlow™ A LOT.  

performance1

FEATURES

The BD OneFlow™ PCD Tube reagent composition

 

AntibodyFluorochromeCloneTarget Populations
CD38FITCHB7Backbone marker. Identification of plasma cells

CD28

PEL293Identification of aberrant plasma cell populations
CD27PerCP-Cy 5.5L128Identificaiton of aberrant plasma cell populations
CD19PE-Cy 7SJ25-C1Backbone marker. Identification of plasma cells
CD117APC104D2Identificaiton of aberrant plasma cell populations
CD81APC-H7JS81Identificaiton of aberrant plasma cell populations
CD45BD Horizon V4502D1Backbone marker. Identification of plasma cells
CD138BD Horizon™ 500-CMI15Backbone marker. Identification of plasma cells

 

The EuroFlow antibody panel article1* has a full description of the utility of the antibodies chosen for the BD OneFlow™ PCD Tube.

 

 

APPLICATIONS

Supporting Evidence

The EuroFlow “PCD panel was able to detect and fully characterize the immunophenotype of aberrant plasma cells or their normal/reactive counterparts in all the samples tested (100), as also assessed by routine diagnostic protocols performed in parallel.”1*

References

  1. van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120

  2. Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779

  3. Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122
For Research Use Only. Not for use in diagnostic or therapeutic procedures.