BD OneFlow™ LST

Overview

The BD OneFlow™ LST is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.¹*

The BD OneFlow™ LST is intended for flow cytometric immunophenotyping of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages in peripheral blood, bone marrow and lymph nodes.

Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ B-CLPD T1, BD OneFlow™ PCST, BD OneFlow™ PCD.

FEATURES

The BD OneFlow™ LST:

Is a preconfigured single-use, ready-to-use 8-color 12-antibodies reagent that is provided in a single-test tube format
Can guide the need for further analysis as a screening tube in combination with panel(s), such as the BD OneFlow™ B-CLPD-T1, specifically designed for the classification of different forms of malignancies (B, T or NK)
Is available in the 20 test/box size (4 pouches of 5 tubes each)
Allows for easy visual identification as dark blue color-coded boxes, pouches and tubes

The BD OneFlow™ Reagents increase efficiency by simplifying instrument standardization, reducing the time to prepare the instrument as well as the technical burden and training needs.²

For BD FACSLyric™ Flow Cytometer users:

BD^® CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility through Universal Setup
BD^® FC Beads (7-Color, 5-Color and 2-Color Kits) support consistency of results, eliminating the need for using cells for compensation
BD^® CS&T Beads and BD^®FC Beads kits necessary for instrument setup and compensation can be used for other CE-IVD assays on the BD FACSLyric™ Flow Cytometer

For BD FACSCanto™ II Flow Cytometer users:

BD FACSDiva™ CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility with Application Settings
BD OneFlow™ Setup Beads ensure data accuracy and reproducibility by providing assay-specific target values, as per EuroFlow™ SOPs³
Specifically designed BD^® FC Beads eliminate the need for using single-vial reagents and cells for compensation

The BD OneFlow™ LST reagent composition

Antibody	Fluorochrome	Clone	Target Populations
CD45	BD Horizon V500-C	2Dl (anti-HLe-1)	Mature lymphocytes, B-cell precursor
CD19	PE-Cy7	SJ25-C1	B-cells, and NK-cells by exclusion
CD20	BD Horizon V450	L27	B-cells, and NK-cells by exclusion
Anti-Lambda	FITC	1-155-2	Normal and clonally expanded B cells
Anti-Kappa	PE	TB28-2	Normal and clonally expanded B Cells
CD38	APC-H7	HB7	Plasma cells and B-cell precursors, Lymphoid malignancies, NK cells
CD3	APC	SK7	T cells, B- and NK-cells by exclusion
CD4	BD Horizon™ 450	SK3 (Leu-3a)	T cell subpopulations
CD8	FITC	SK1 (Leu-2a)	T cell subpopulations
CD5	PerCP-Cy5.5	L17F12	T cell subpopulations
Anti-TCR-1	PE-Cy7	11F2	T cell subpopulations
CD56	PE	MY31 (Leu-19)	NK Cells

The EuroFlow antibody panel article¹* has a full description of the utility of the antibodies chosen for the BD OneFlow™ LST.

APPLICATIONS

BD OneFlow™ LST Lab Report (normal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of a normal bone marrow specimen stained with the 8-color 12-antibody BD OneFlow™ LST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages.

BD OneFlow™ LST Lab Report (abnormal specimens) when used with the BD FACSLyric™ Flow Cytometer

Laboratory report of an abnormal bone marrow specimen stained with the 8-color 12-antibody BD OneFlow™ LST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages.

BD OneFlow™ Reagents can be run on both the BD FACSLyric™ and BD FACSCanto™ II Flow Cytometers with equivalent results.

Supporting Evidence

The EuroFlow “LST detected aberrant B-, T-, or NK-cells immunophenotypes in 149/150 (99.4%) of B-CLPD and in 78/83 (94%) of T/NK-CLPD with an overall frequency of 97.4%."¹*

Results from the clinical trial study show that the BD OneFlow™ LST in combination with the BD OneFlow™ B-CLPD T1 gave 100% (101 of 101) overall agreement in classifying patients as having CLL (54 of 54 concordant) and in identifying patients with other B cell chronic lymphoproliferative diseases (47 out of 47 concordant) when compared with the EuroFlow system. Furthermore, interpretation of the BD OneFlow™ Solution results was highly concordant to diagnostic truth.

RESOURCES

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Instructions for Use

BD OneFlow™ Setup Beads (BD FACSCanto™ II Flow Cytometer)

BD^® FC Beads 8-Color Kit for BD OneFlow™ Assays (BD FACSCanto™ II Flow Cytometer)

Tools

BD Antibodies in EuroFlow™ Multicolor Panels

EuroFlow™ Multicolor Panels

References

van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120
Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779
Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122