BD Rhapsody™ Express Single-Cell Analysis System

• Minimal set of benchtop equipment, no fluidic pumps
• Allows gentle treatment of cells as they settle into microwells by gravity
• Each BD Rhapsody™ Cartridge contains a microwell array with >220,000 partitions
• The BD Rhapsody™ Cartridge has a volume ~600 µL; dilute cell suspensions can be loaded directly into the cartridge without needing to concentrate them into very small volumes
• No microfluidic channels are involved so clogging of channels with cells is less of a concern
• The microwells are specially treated to prevent cells from adhering to surfaces and extensive QC is performed

• BD Rhapsody™ Beads remain intact throughout the workflow
• Beads with cDNA molecules can be stored for several months
• Beads can be subsampled for creation of multiple sequencing libraries
• Archived beads can be pooled and sequenced together for operational efficiency
• Archived beads can be shared easily with research partners
• Archived, subsampled beads can help standardize performance and develop SOPs

High correlation in gene expression and minimal batch effects seen in data obtained from beads stored up to 12 weeks.

Reliable, reproducible results from gentle, gravity-based single-cell partitioning without microfluidics

• Minimal batch effects with technical replicates
• Minimal batch effects with biological replicates
• Minimal site-to-site variability
• Minimal user-to-user variability
• Minimum sample bias
• Ability to integrate large data sets without correction for batch effects

High correlation in gene expression seen between technical and biological replicates and between users and sites.