BD FACSAria™ III Cell Sorter

A sensitive flow cytometer with dependable performance

Overview

The BD FACSAria™ III Cell Sorter is built on the solid foundation of patented technologies, superior multicolor performance and legendary ease-of-use that opened the complex world of cell sorting to a broader audience of researchers and wider range of research applications.

The fluidics and optical systems are precisely integrated to maximize signal detection. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve high sensitivity and resolution.

Get more information from the BD FACSAria™ III Sorter brochure.

FEATURES

The BD FACSAria™ III Sorter enables high-performance analysis and high-performance sorting

Through the precise coordination of the optical and fluidics systems, the BD FACSAria™ III System delivers exceptional optical detection sensitivity

Fixed alignment of the cuvette flow cells and the laser minimizes startup time, improves experiment-to-experiment reproducibility, reduces operator-to-operator variability and enables automated daily quality control
Innovations in the fluidics system such as easy-to-insert nozzles and easy-to-change filters make setup fast and simple
Software wizards in BD FACSDiva^™Software make aseptic sort setup easy and effective
BD FACS™ Accudrop technology simplifies drop-delay determination

Get more information about the optics, fluidics and electronics of the BD FACSAria™ III Sorter from the system brochure.

The BD FACSAria™ III Sorter provides increased efficiency for multicolor detection, flexibility to expand and lower cost of ownership

The BD FACSAria™ III Sorter’s optical system efficiently maximizes signal detection and greatly increases sensitivity and resolution for each color in a multicolor assay

Enhanced sensitivity and resolution ensure that even dim populations can be readily identified and sorted
The system can support five lasers and four spatially separated beam spots. Choose up to six laser wavelengths—633, 561, 488, 405, and 355 nm—and up to 20 detector positions, to measure up to 18 colors simultaneously
The system can be expanded with additional lasers via a field upgrade. You can design a configuration that meets your lab’s budget and site requirements today and have a growth path for the future

APPLICATIONS

The BD FACSAria™ III Sorter has been successful in identifying both undifferentiated and differentiated pluripotent stem cells.

Differentiating H9 cells. A combination of three different fluorescent antibodies were used to identify both undifferentiated (TRA-1-81 and SSEA-3) and differentiated (SSEA-1) pluripotent stem cells. This combination of markers has been widely used to characterize and isolate differentiated and undifferentiated stem cells derived from hESCs and iPS cells.

The BD FACSAria™ III Sorter has been used in sorting Treg cells.

Representative FoxP3 staining of sorted Tregs. To determine purity, as defined by FoxP3+ status, a portion of the cells was stained with an anti-human FoxP3 antibody conjugated to the BD Horizon™ V450 Dye. Data is representative of 10 experiments.