For Professionals in Research
Overview
The BD Special Order Research Products (SORP) program lets customers rapidly configure BD flow cytometers (both cell analyzers and sorters) to fit precise research needs. Designed and configured in collaboration with customers, BD special order products offer a wide range of choices to help researchers create the ultimate customized instrument tailored to the special needs of research at the leading edge of biomedical discovery.
Special Order Research Product Overview
BD special order sorters and analyzers offer advanced and flexible features available for multicolor research
To support advanced assay development, BD special order instrumentation deliver the latest in laser technology to researchers to achieve a wide range of color, wavelength and power choices.
The special-order systems are designed and configured in collaboration with customers to meet exact research requirements.
Getting started
Your BD Sales Representative is your point of contact to discuss a special-order product for your lab. Together with an engineer from the Special Order team, they will follow a structured process to define, design and manufacture an instrument that exactly matches your requirements.
Measurement of the antigen expression of human bone marrow cells
Seven-laser excitation of seven fluorochromes is shown (right), demonstrating the utility of a seven-laser special order BD® LSR II Analyzer.*
Each spatially separated beam provides discrete fluorochrome excitation so that virtually no computer compensation is required for complete cell population separation. Data courtesy of Frederic Preffer (Harvard Medical School, Boston MA). Reference: Preffer F, Dombkowski D. Advances in complex multiparameter flow cytometry: Applications in stem cell research. Cytometry B Clin Cytom. 2009;76(5):295-314. doi: 10.1002/cyto.b.20480
*The BD® LSR product family includes the special order BD® LSR II and BD LSRFortessa™ Analyzers. This experiment was run on a BD® LSR II Analyzer configured with the following lasers: 20-mW 355-nm UV, 50-mW 405-nm violet, 20–100 mW 488-nm blue (variable power), 150-mW 532-nm green (variable power), 50-mW 594-nm yellow, 40-mW 638-nm red, and 25-mW 785-nm IR.
Probability state model of human B-cell maturation
Human bone marrow was stained with CD23 Pacific Blue™, CD20 AmCyan, TdT FITC, CD34 PerCP, CD43 PE, CD19 PE-Cy5.5, CD10 PE-Cy7, Kappa APC, CD45 APC-Cy7, and Lambda Alexa Fluor™ 594. Approximately 10,000 cells were analyzed. The percentage of cells in each state was plotted against the level of antigen expression measured. B-cell precursors are seen to be downregulating early markers such as CD34 and TdT and upregulating more mature antigens such as CD20, CD23 and surface immunoglobulin light chain. Analysis by GemStone™ Software (Verity Software House, Topsham, ME). Data courtesy of Frederic Preffer (Harvard Medical School, Boston MA). Reference: Preffer F, Dombkowski D. Advances in complex multiparameter flow cytometry: Applications in stem cell research. Cytometry B Clin Cytom. 2009;76(5):295-314. doi: 10.1002/cyto.b.20480
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
