![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
Old Browser
Overview
The BD® ELISPOT Assay is a powerful tool for detecting and analyzing individual cells that secrete a particular protein in vitro. BD® ELISPOT Reagents coupled with a high-throughput ELISPOT plate reader provide intuitive analysis of cytokines at the single-cell level.
The reagents:
- Provide direct analysis of frequency of cells producing cytokines and other effector molecules using an enzyme-linked immunospot (ELISPOT) assay
- Offer greater sensitivity than ELISAs and intracellular staining
- Enable high-throughput analyses for direct ex vivo monitoring of T-cell function, including antigen-specific T cells
- Offer the flexibility to choose among several formats:
- BD® ELISPOT Kits—ready-to-use kits with precoated BD® ELISPOT Plates for human, nonhuman primate and mouse research
- BD® ELISPOT Reagent Sets—coat-it-yourself sets with sufficient capture antibody, detection antibody and streptavidin-horseradish peroxidase for 10 plates worth of assays (includes 10 uncoated plates)
- BD® ELISPOT Reagent Pairs–matched antibody pairs. Sufficient capture antibody and detection antibody for 5 plates
![](https://dev-gl.bdbiosciences.com/content/dam/bdb/products/reagents/ELISPOT-Overview-Image.jpg)
Features
The BD® ELISPOT Assay is able to detect cytokine release at the single-cell level, allowing for direct determination of cytokine-producing cell frequencies
The assay:
- Has been found to be more sensitive than ELISAs and intracellular staining. The sensitivity of the assay enables measurement of very low frequencies of cytokine-producing cells (for example, 1 in 300,000).
- Enables single-cell and high-throughput analyses for direct ex vivo monitoring of T-cell function, including antigen-specific T cells.
- Contains carefully screened reagents to ensure the generation of crisp, clearly defined immunospots. High-affinity BD Pharmingen™ NA/LE Capture Antibodies safeguard against nonspecific cellular activation.
- Has carefully selected detection antibodies, along with ready-to-use preparations of streptavidin-horseradish peroxidase, substrate and chromogen to produce optimal BD® ELISPOT Reagents.
An automated coating process for the BD® ELISPOT Plates helps to minimize inconsistency of results
BD Biosciences kits undergo strict quality control processes to ensure high-quality assay results with minimal well-to-well and plate-to-plate variation. The plates are coated with no azide/low endotoxin capture antibodies, are UV processed, and are tested and found negative for bacteria and fungi.
![](/etc.clientlibs/bdb-aem/clientlibs/clientlib-base/resources/icons/blankimage.png)
-
Frequently Asked Questions
-
Product Information Sheets
-
Product List
-
Protocols
-
Tools
-
Blocking the Plate
-
Capture Antibody Preparation and Coating
-
Getting Started
-
Standard Preparation and Handling
-
Standard and Sample Addition
-
Stopping the Reaction and Reading the Plate
-
TMB Substrate Preparation and Addition
-
Troubleshooting: High Background
-
Troubleshooting: Low/No Signal
-
Troubleshooting: Poor Standard Curve
-
Troubleshooting: Well-to-Well Precision
-
Washing the Plates
-
Working Detector Preparation
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
QuickTime is a trademark of Apple Inc. Windows Media is a trademark of Microsoft Corporation.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.