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      BD FACSymphony™ A3 Cell Analyzer

      A customizable flow cytometer that provides high-parameter analysis in a smaller footprint

      Overview

      The BD FACSymphony™ A3 Cell Analyzer:

      • Enables the simultaneous detection of up to 30 parameters and 28 colors
      • Offers highly customizable options to meet your research priorities and can be configured to meet your needs today with room for growth tomorrow
      • Harnesses the power of the full BD solution to reach deeper insights with state-of-the-art analyzers, a portfolio of bright dyes, specialized technical support and high-dimensional analysis tools
      • Provides the flexibility to match your needs and budgets
         

      Contact your local representative to discuss the ideal instrument to meet your needs.

       

      Learn more from the BD FACSymphony™ A3 Cell Analyzer brochure.

       
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      Features and capabilities of the BD FACSymphony™️ A3 and its counterpart BD FACSymphony™️ A5 Cell Analyzers

      BD FACSymphony™
      A3 Cell Analyzer      		 BD FACSymphony™
      A5 Cell Analyzer      		 BD FACSymphony™
      A5 Cell Analyzer-HPC Option
      Analyze √ √ √
      VPX electronics √ √ √
      Maximum lasers 5 9 9
      Maximum parameters 30 50 50
      Array type Decagon Decagon HP Cascade
      Maximum detectors per laser 10 10 20
      Detector type Tube PMT Tube PMT High-sensitivity square PMT
      Discrete pre-amps √
      Exchangeable filters √ √ √
      HTS option √ √ √
      FFSS option √ √ √


      Find out more about the BD FACSymphony™ A5 Cell Analyzer.

      The BD FACSymphony™ A3 Cell Analyzer improves sensitivity to enable you to identify and analyze rare cell types and events.

      The instrument features an ultra-quiet VPX electronics system that supports up to 30 high-performance detectors.

      The capabilities of this platform technology uniquely allow you to conduct deep and broad phenotyping and gain richer scientific insights by fully leveraging the broad portfolio of catalog and custom BD Horizon Brilliant™ Reagents.

      As a Special Order Research Product (SORP), the BD FACSymphony™ A3 Cell Analyzer offers a choice of lasers from 26 different laser wavelengths to optimally configure your instrument for your specific research application. 

      For most lasers, multiple power ratings can be adjusted, stored and recalled using the digital laser command and control functionality are available.

      Dependent on the laser, power settings from 20–1,000 mW may be available.

      Fluorochrome availability and excitation characteristics across various wavelengths should be discussed during the configuration process to identify the best optical options for your research.

      The BD FACSymphony™ A3 Cell Analyzer allows you to configure up to 5 lasers and provides the flexibility to implement new lasers and detectors later with field upgrades

      Lasers

      • Select up to five lasers for optimization of your research needs 
      • Configure your instrument using traditional or unique wavelengths and power ratings

      Optical arrays

      • Photomultiplier tubes (PMTs) in decagon arrays with up to 10 detectors per laser line
      • Filter sets exchangeable for traditional fluorescent markers as well as specialized sets for unique dyes or proteins

      The BD® High Throughput Sampler (HTS) and BD FACSFlow™ Supply System (FFSS) options are available for increased efficiency

      The HTS option:

      • Automates and accelerates sample acquisition
      • Compatible with 96- and 384-well plates
      • <0.5% sample carryover in high-throughput mode

      The FFSS option:

      • Increases capacity and ease of use while maintaining a stable fluidics pressure
      • Reduces daily maintenance by incorporating a 20-L BD FACSFlow™ Cubitainer
      Laser Wavelength (nm) Power (mW)
      UV 355 20-100
      Violet 405 100-200
      Blue 488 100-200
      Yellow Green 561 100-200
      Red 637 140


      Find out more about the BD FACSymphony™ A5 Cell Analyzer.

      PERFORMANCE

      The BD FACSymphony™ A3 Cell Analyzer works with conventional and BD Horizon Brilliant™ Fluorochromes to expand experimental design and help accelerate time to insight.

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      Enable on-site training and instrument characterization with CD4 fluorochrome evaluation kits

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      Reduce cost and risk of optimizing new high-parameter panels

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      High-dimensional data analysis

      The BD FACSymphony™ A3 Cell Analyzer and FlowJo™ v10 Software enable high-dimensional data analysis

       

      Take your analysis to the next level with new features in FlowJo™ v10 Software including improved BD FACSDiva™ Software support, platform overlays, spectral compensation and more.

       

      Useful tools for high-dimensional visualization and analysis of BD FACSymphony™ Cell Analyzer data include t-SNE, OptSNE, UMAP, TriMAP and EmbedSOM.

       

      Keep up to date on the latest plugins at FlowJo Exchange.

      APPLICATIONS

      The BD FACSymphony™ A3 Cell Analyzer was used in deep immunophenotyping of human circulating dendritic cells.

      Deep immunophenotyping of human circulating dendritic cells using an 18-color flow cytometry panel: A) Identification of major dendritic cell (DC) subsets from human healthy peripheral blood mononuclear cells. After exclusion of CD3+, CD56+ and CD19+ lineage cells, DCs are identified and gated as HLA-DR+ CD14– cells. From this gate, CD141+ cDC1 (blue) and CD16+ DCs (green) are identified. From the CD141– CD16– gate, AXL+ DCs are defined as AXL+ Siglec6+ cells. AXL+ DCs are further divided into CD123+ (violet) and CD11c+ (orange) Axl+ DCs. From the Axl– Siglec6– gate, CD123+ CD11c– pDCs (aqua) and CD11c+ CD1c+ cDC2 (red) are identified. B) The 18-color panel further enabled the analysis of eight additional DC phenotyping markers and the identification of distinct expression patterns across the major DC subsets. Samples were acquired on a BD FACSymphony™ A3 Cell Analyzer.

      Class 1 Laser Product.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Note: Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. 

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