Protocols

Expand All

Apoptosis

Induction FAS

Induction DX2

Death Staurosporine

Cell Death

Annexin V Staining Protocol

APO-BRDU™ Procedure

APO-DIRECT™ Procedure

Annexin V with Cell Surface Antibody Staining for Suspension Cells

Annexin V Staining of Adherent Cells for Flow Cytometry

Cell Biology

Immunoprecipitation with Antibody: Agarose Conjugates

Immunoprecipitation with Anti-Phosphotyrosine: Biotin Conjugates

Immunoprecipitation with Soluble Antibodies

Western Blotting with Rabbit Polyclonal Antibodies

Western Blotting with Monoclonal Antibodies

Bioimaging in 96 well plates

Western Blotting with Biotinylated Antibodies

Immunoprecipitation with Antibody: Agarose Conjugates

Western Blotting with Horseradish Peroxidase Conjugates or Alkaline Phosphatase Conjugates

Cell Cycle

Human Cyclins

Detection Ki-67

BrdU Detection

Flow Cytometry

Procedure for Manual Setting of Compensation for Multicolor Flow Cytometry Cytometric Analysis

Uses of BD Fc Block™ Reagent

Stain Lyse Wash

Stain Lyse No Wash

BD PhosFlow™ Buffer Cytometry Protocols for Mouse Splenocytes or Thymocytes

Mouse and Rat Leukocytes

Isotope Control

Indirect Staining Mononuclear Cells

Immunopurification of Tyrosine Phosphorylated Proteins

BD PhosFlow™ Buffer Cytometry Protocol for Human PBMCs

BD PhosFlow™ Buffer Cytometry Protocol for Human Whole Blood Samples

The Escapee Phenomenon

Fix Perm Kits

Protocol Direct Immunofluorscence Staining

Cytokines FCA

Cell Surface

Cytokine Stimulation and 96 Well BD Phosflow™ Buffer Protocol

TCR Stimulation and 96 Well BD Phosflow™ Buffer Protocol

Activation Immune Cells

BD Phosflow™ Buffer Protocol for Adherent Cells

Preparation and Staining of Paraffin Sections

Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes

Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs

Generation of Human IL-17–Producing Cells (Th17-like Cells) In Vitro

Live Cell Discrimination and Measurement of Phosphorylation and Cell Surface Markers in Thawed and Activated Human PBMCs

Freezing and Thawing of Peripheral Blood Mononuclear Cells (PBMCs)

Preparation of Murine Splenocytes

Tissue Preparation for Surface Antigen Staining

Bulk Erythrocyte Lysing with Ammonium Chloride for Flow Cytometry Cytometry Immunophenotyping

IHC/IF

Immunohistochemistry/Tissue Section Staining

Cell Staining for Immunofluorescence Microscopy

Immunohistochemical Staining Method for Phosphoproteins in Paraffin Sections

Immunofluorescence

Preparation and Staining of Frozen Tissue Sections

Preparation and Staining of Paraffin Sections

Fix Perm Kits

Protocol Direct Immunofluorescence Staining

BD OptEIA™ Kits

Mouse IgE ELISA Protocol

Cytokine ELISA Protocol

RBC/Platelet

Surface Staining

Platelet Activation

Intracellular Staining

Indirect Immunofluorescence

Single-Cell Multiomics

BD Rhapsody™ Targeted mRNA & AbSeq Amp Kit

BD Rhapsody™ WTA Amplification Kit

BD Rhapsody™ Targeted mRNA AbSeq Kit 4 Pack (sales BOM)

BD Rhapsody™ WTA Reagent Kit – 4 pack (sales BOM)

Hu Single Cell Sample Multiplexing Kit

Ms Single Cell Sample Multiplexing Kit

BD Rhapsody™ Immune Response Panel HS

BD Rhapsody™ T cell Expression Panel HS

BD Rhapsody™ Onco-BC Panel HS

BD Rhapsody™ Immune Response Panel Mm

BD Rhapsody™ Panel Supplement

BD Rhapsody™ Custom Panel

BD® AbSeq Ab-Oligos (all SKU's)

VDJ CDR3 Protocols

BD Rhapsody™ Cartridge Reagent Kit

BD Rhapsody™ Cartridge Kit

BD Rhapsody™ cDNA Kit

Stem Cell

Cell Surface Staining of Stem Cell Lines and Other Adherent Cells for Flow Cytometry Cytometry

Live Cell Discrimination and Measurement of Phosphorylation and Cell Surface Markers in Thawed and Activated Human PBMCs

Freezing and Thawing of Peripheral Blood Mononuclear Cells (PBMCs)

Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs

Preparation of Murine Splenocytes

Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.