ROCK Inhibitor (Y-27632)

BD™ ROCK Inhibitor (Y-27632)

Name: ROCK Inhibitor (Y-27632)
Brand: BD™
SKU: 562822

Protocol Library

Scientific Resources

Protocol Library

Scientific Resources

Product Details

Brand: BD™

Alternative Name: Y-27632 dihydrochloride

Application: Cell culture (Tested During Development)

RRID: AB_2869435

Preparation And Storage

Store at room temperature, desiccated and protected from prolonged exposure to light. Store aliquots of the reconstituted material at -20°C, protected from prolonged exposure to light, and avoid repeated freeze-thaw cycles.

Recommended Assay Procedures

For a working solution, dilute to 10 mM in sterile deionized water. [The molar mass of Y-27632 is 338.3 g/mol.]

Small aliquots may be stored at -20°C, avoiding repeated freeze-thaw cycles.

Use at 10 µM in culture medium. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Avoid contact with skin and eyes.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
mTESR™1 is a trademark of StemCell Technologies.
Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Companion Products

Human Pluripotent Stem Cell Sorting And Analysis Kit

Size 50 Tests Cat No. 560461

Accutase™ Cell Detachment Solution

Size 100 mL Cat No. 561527

562822 Rev. 1

Antibody Details

The ROCK inhibitor, Y-27632, is a selective inhibitor of the p160-Rho-associated coiled kinase (ROCK) and is a factor that enhances embryonic stem cell survival upon single cell dissociation. In addition to increasing cell recovery after dissociation, Y-27632 has been used for various other applications in stem cell research including cryopreservation, sorting, reprogramming, transplantation, and differentiation.

562822 Rev. 1

Citations & References

View product citations for antibody "562822" on CiteAb

Development References (5)

Braam SR, Nauw R, Ward-van Oostwaard D, et al. Inhibition of ROCK improves survival of human embryonic stem cell-derived cardiomyocytes after dissociation. Ann N Y Acad Sci. 2010; 1188:52-57. (Methodology: Cell culture). View Reference
Emre N, Vidal JG, Elia J, et al. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. PLoS ONE. 2010; 5(8):e12148. (Methodology: Cell culture). View Reference
Li X, Meng G, Krawetz R, Liu S, et al. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation. Stem Cells Dev. 2008; 17(6):1079-1085. (Methodology: Cell culture). View Reference
Narumiya S, Ishizaki T, Uehata M. Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 2000; 325:273-284. (Biology: Cell culture). View Reference
Watanabe K, Ueno M, Kamiya D. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol. 2007; 25(6):681-686. (Methodology: Cell culture). View Reference

View All (5)

562822 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.