BD Horizon Red 718

BD Horizon™ Red 718 Reagents

BD Horizon™ Red 718 (R718) is an innovative small-molecule fluorochrome developed exclusively for BD Biosciences. It provides improved sensitivity and resolution of low-density markers and intracellular targets. R718 is excited by the red laser with an emission maximum of 718 nm and can be detected by the same filter as Alexa Fluor™ 700.

BD Horizon™ Red 718 Reagents are specially designed to serve as alternatives to Alexa Fluor™ 700 or APC-R700.

BD Horizon™ Red 718 Reagents offer:

Greater brightness and resolving power than Alexa Fluor™ 700 for both surface and intracellular markers
Reduced background staining compared to Alexa Fluor™ 700 and APC-R700
Minimal spread into the APC channel
A broad portfolio of conjugates through BD OptiBuild™ On-Demand Reagents

BD Horizon™ Red 718 Reagents have the potential to enhance your flow cytometry panel's performance.

Product Information Sheet Available Reagents

BD Horizon™ Red 718 Reagents

Performance

The CD4+IL-17+ cells are better resolved with BD Horizon™ Red 718 than Alexa Fluor™ 700.

Two-color comparison staining of IL-17A on stimulated human peripheral blood lymphocytes.

Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate and Ionomycin in the presence of BD GolgiStop™ Protein Transport Inhibitor. The cells were permeabilized with BD Perm/Wash™ Buffer then stained with BD Horizon™ R718 Mouse Anti-Human IL-17A (clone N49-653, A) or BD Pharmingen™ Alexa Fluor™ 700 Mouse Anti-Human IL-17A (clone N49-653, B) and BD Pharmingen™ PE Mouse Anti-Human CD4 (clone RPA-T4) shown as two-color flow cytometric pseudocolor density plots derived from gated lymphocytes and were analyzed the same day on a BD LSRFortessa™ X-20 Cell Analyzer with FlowJo™ Software.

Brightness

BD Horizon™ R718 Reagent shows superior brightness and resolution as compared to Alexa Fluor® 700.

Comparison staining of CD4 on human lysed whole blood. A. Lysed whole blood stained with BD Horizon™ R718 Mouse Anti-Human CD4 (clone SK3, red) or BD Pharmingen™ Alexa Fluor™ 700 Mouse Anti-Human CD4 (clone SK3, grey) shown overlaid as fluorescence histograms derived from a lymphocyte gate. B. SSC vs. fluorescence plots of BD Horizon™ R718 Mouse Anti-Human CD4 (left) or BD Pharmingen™ Alexa Fluor™ 700 Mouse Anti-Human CD4 (right). All data were analyzed on a BD LSRFortessa™ X-20 Cell Analyzer with FlowJo™ Software

Flexibility

BD Horizon™ R718 resolves difficult intracellular markers, expanding the capabilities of the 700 nm detector.

A. BD Horizon™ R718 FoxP3 is on par with the gold standard BD Pharmingen™ PE FoxP3. Human PBMCs were stained with BD Horizon™ R718 Mouse Anti-Human FoxP3 (clone 259D/C7). B. BD Phosflow™ Treated Human Control Cells (red) and BD Phosflow™ Untreated Human Control Cells (grey) were stained with BD Phosflow™ R718 Mouse Anti-Stat5 (pY694) (clone 47); treated and untreated control cell overlays shown here are derived from a CD4+ gate.

BD OptiBuild™ On-Demand R718 Reagents Offer Superior Resolution

Comparison of BD Horizon™ R718 Reagents (red), BD OptiBuild™ On-Demand Red 718 Reagents equivalent (blue), vs. BD Pharmingen™ Alexa Fluor™ 700 (orange) using surface markers mouse anti-human CD3 (left, clone UCHT1) or rat anti-mouse CD8a (right, clone 53-6.7)

Featured Reagents

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Custom Reagents or Bulk Orders

Reagent Specificity	Species	Clone	Isotype	Size	Catalog Number
BCL-6	Human	K112-91	Mouse IgG1, κ	100 Tests	566979
CD3	Human	UCHT1	Mouse IgG1, κ	100 Tests	566953
CD3	Human	UCHT1	Mouse IgG1, κ	25 Tests	566954
CD4	Human	SK3	Mouse IgG1, κ	100 Tests	566930
CD4	Human	SK3	Mouse IgG1, κ	25 Tests	566931
CD11c	Human	B-ly6	Mouse IgG1, κ	100 Tests	566932
CD11c	Human	B-ly6	Mouse IgG1, κ	25 Tests	566933
CD19	Human	SJ25C1	Mouse IgG1, κ	100 Tests	566946
CD19	Human	SJ25C1	Mouse IgG1, κ	25 Tests	566947
CD279 (PD-1)	Human	EH12.1	Mouse IgG1, κ	100 Tests	566974
CD279 (PD-1)	Human	EH12.1	Mouse IgG1, κ	25 Tests	566975
FoxP3	Human	259D/C7	Ms IgG1	100 Tests	566935
FoxP3	Human	259D/C7	Ms IgG1	25 Tests	566936
IL-17A	Human	N49-653	Mouse IgG1, κ	100 Tests	566938
Stat5 (pY694)	Human	47/Stat5 (pY694)	Mouse IgG1, κ	100 Tests	566977
Stat5 (pY694)	Human	47/Stat5 (pY694)	Mouse IgG1, κ	25 Tests	566978
CD107a	Mouse	1D4B	Rat IgG2a, κ	50 ug	566986
CD4	Mouse	RM4-5	Rat IgG2a, k	50 ug	566939
CD8a	Mouse	53-6.7	Rat IgG2a, κ	50 ug	566985
CD13	Mouse	R3-242	Rat IgG1	50 ug	566982
Ly-6C	Mouse	AL-21	Rat IgG2a, κ	50 ug	566987

Switch to BD Horizon™ Red 718 Reagents to achieve your resolution goals.

Available Reagents

23-22638-00

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.