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AbSeq Protocols
Cell Labeling
- This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides).
- Doc ID: 214394 Rev. 1.0
- Revision date: 07/2018
- This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(1 plex to 40 plex) with the BD® Single-Cell Multiplexing Kit.
- Doc ID: 214419 Rev. 2.0
- Revision date: 02/2019
- This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit.
- Doc ID: 23-22354-00
- Revision date: 05/2020
Sequencing Library Preparation
- This protocol provides instructions on creating mRNA and BD™ AbSeq single cell libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
- Doc ID: 214293 Rev.3.0/23-21343-00
- Revision date: 02/2019
- This protocol provides instructions on creating single cell mRNA, Sample Tag, and BD™ AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
- Doc ID: 214508 Rev.3.0/23-21335-00
- Revision date: 02/2019
- This protocol provides instructions on creating single cell whole transcriptome mRNA and AbSeq protein detection libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
- Doc ID: 23-21751-00
- Revision date: 07/2019
- This protocol provides instructions on creating single cell whole transcriptome mRNA, AbSeq, and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers.
- Doc ID: 23-21752-00
- Revision date: 07/2019
- This protocol provides instructions on creating single-cell mRNA and BD Abseq libraries with the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. It is also intended to provide a method to enable a high-throughput detection of VDJ recombination events by generating sequencer-ready libraries specific to the CDR3-specific antigen binding regions of B and T cells.
- Doc ID: 23-22203-01
- Revision date: 05/2020
- This protocol provides instructions on creating single-cell mRNA, Sample Tag, and BD Abseq libraries with the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. It is also intended to provide a method to enable a high-throughput detection of VDJ recombination events by generating sequencer-ready libraries specific to the CDR3-specific antigen binding regions of B and T cells for human samples.
- Doc ID: 23-22204-01
- Revision date: 05/2020
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