Product Details
BD FastImmune™
Human
Flow cytometry
Phosphate buffered saline with gelatin and 0.1% sodium azide.
Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
340456 Rev. 2
Components
| Description | Clone | Isotype | |
|---|---|---|---|
| IL-4 PE | 1A8 | IgG2a, κ | N/A |
| IFN-γ FITC | TgMab-2 | IgG1, κ | N/A |
340456 Rev. 2
Citations & References
Development References (15)
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Aggarwal B, Puri R. Human Cytokines: Their Role in Disease and Therapy. Cambridge, MA: Blackwell Science; 1995:3-24.
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Bradding P, Feather IH, Howarth PH, et al. Interleukin 4 is localized to and released by human mast cells. J Exp Med. 1992; 176:1381-1386. (Biology: Flow cytometry).
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology: Flow cytometry).
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Clinical and Laboratory Standards Institute. 2005. (Biology: Flow cytometry).
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ElGhazali GEB, Paulie S, Andersson G, et al. Number of interleukin-4– and interferon- γ –secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutindistinct response profiles depending on the type of antigen used for activation. Eur J Immunol. 1993; 23:2740-2745. (Biology: Flow cytometry).
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Elliott MJ, Gamble JR, Park LS, Vadas MA, Lopez AF. Inhibition of human monocyte adhesion by interleukin-4. Blood. 1991; 77:2739-2745. (Biology: Flow cytometry).
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Hardy KJ, Sawada T. Human γ interferon strongly upregulates its own gene expression in peripheral blood lymphocytes. J Exp Med. 1989; 170:1021-1026. (Biology: Flow cytometry).
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Idzerda RL, March CJ, Mosley B, et al. Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J Exp Med. 1990; 171(3):861-873. (Biology: Flow cytometry). View Reference
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Johnson HM, Bazer FW, Szente BE, Jarpe MA. How interferons fight disease. Scientific American. 1994; May:68-75. (Biology: Flow cytometry).
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Openshaw P, Murphy EE, Hosken NA, et al. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med. 1995; 182(5):1357-1367. (Biology: Flow cytometry). View Reference
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Paliard X, Malefijt RDW, Yssel H, et al. Simultaneous production of IL-2, IL-4, and IFN-γ by activated human CD4+ and CD8+ T cell clones. J Immunol. 1988; 141:849-855. (Biology: Flow cytometry).
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Paul WE. Interleukin-4: a prototypic immunoregulatory lymphokine. Blood. 1991; 77(9):1859-1870. (Biology: Flow cytometry). View Reference
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Powrie F, Coffman RL. Cytokine regulation of T-cell function: potential for therapeutic intervention. Immunol Today. 1993; 14:270-274. (Biology: Flow cytometry).
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Romagnani S, Del Prete G, Maggi E, et al. Human TH1 and TH2 subsets. Int Arch Allergy Immunol. 1992; 99:242-245. (Biology: Flow cytometry).
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Street NE, Mosmann TR. Functional diversity of T lymphocytes due to secretion of different cytokine patterns. FASEB J. 1991; 5:171-176. (Biology: Flow cytometry).
340456 Rev. 2
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
