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      Anti-Human CD45RA FITC/CD62L PE/CD3 PerCP/CD4 APC

      BD Multitest™ Anti-Human CD45RA FITC/CD62L PE/CD3 PerCP/CD4 APC

      (RUO (GMP))
      Product Details
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      BD Multitest™
      Human
      Flow cytometry
      RUO (GMP)
      Phosphate buffered saline with BSA and 0.1% sodium azide.


      Preparation And Storage

      The MultiTEST™ reagent is supplied as a combination of CD45RA FITC, CD62L PE, CD3 PerCP, and CD4 APC in 1.0 mL of phosphate-buffered saline (PBS) containing bovine serum albumin and 0.1% sodium azide. Store vials at 2–8°C. Do not freeze; protect from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      340977 Rev. 2
      Components
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      Description Clone Isotype
      CD62L PE N/A N/A N/A
      CD4 APC N/A , N/A
      CD3 PerCP HI10a IgG1, κ N/A
      CD45RA FITC RM4-5 IgG2a, κ N/A
      340977 Rev. 2
      Citations & References
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      Development References (35)

      1. Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:9-108.
      2. Bevilacqua M, Butcher E, Furie B, et al. Selectins: a family of adhesion receptors. Cell. 1991; 67:233. (Biology: Flow cytometry).
      3. Brenner M, Groh V, Porcelli A, et al. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:1049-1053.
      4. Camerini D, James SP, Stamenkovic I, Seed B. Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor. Nature. 1989; 342(6245):78-82. (Biology: Flow cytometry). View Reference
      5. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology: Flow cytometry).
      6. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
      7. Dalgleish AG, Beverley PC, Clapham PR, Crawford DH, Greaves MF, Weiss RA. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.. Nature. 312(5996):763-7. (Biology: Flow cytometry). View Reference
      8. Diacovo T, Springer TA. CD62L (L-selectin) cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1503-1504.
      9. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology: Flow cytometry). View Reference
      10. Garson JA, Beverley PCL, Coakham HB, Harper EJ. Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982; 298:375-377. (Biology: Flow cytometry).
      11. Gatenby PA, Kansas GS, Xian CY, Evans RL, Engleman EG. Dissection of immunoregulatory subpopulations of T lymphocytes within the helper and suppressor sublineages in man. J Immunol. 1982; 129(5):1997-2000. (Biology: Flow cytometry). View Reference
      12. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
      13. Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology: Flow cytometry).
      14. Kanof ME, James SP. Leu-8 antigen expression is diminished during cell activation but does not correlate with effector function of activated T lymphocytes. J Immunol. 1988; 140:3701-3706. (Biology: Flow cytometry).
      15. Kanof ME, Strober W, James SP. Induction of CD4 suppressor T cells with anti-Leu-8 antibody. J Immunol. 1987; 139(1):49-54. (Biology: Flow cytometry). View Reference
      16. Kansas GS, Wood GS, Engleman EG. Maturational and functional diversity of human B lymphocytes delineated with Anti-Leu-8. J Immunol. 1985; 134:3003-3006. (Biology: Flow cytometry).
      17. Kansas GS, Wood GS, Fishwild DM, Engleman EG. Functional characterization of human T lymphocyte subsets distinguished by monoclonal anti-leu-8. J Immunol. 1985; 134(5):2995-3002. (Biology: Flow cytometry). View Reference
      18. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
      19. Kurrle R. Knapp W, ed. Leucocyte Typing IV. New York, NY: Oxford University Press; 1989:290-293.
      20. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986; 137(8):2501-2507. (Biology: Flow cytometry). View Reference
      21. Lanier LL, Engleman EG, Gatenby P, Babcock GF, Warner NL, Herzenberg LA. Correlation of functional properties of human lymphoid cell subsets and surface marker phenotypes using multiparameter analysis and flow cytometry. Immun Rev. 1983; 74:143. (Biology: Flow cytometry).
      22. Lanier LL, Loken MR. Human lymphocyte subpopulations identified by using three-color immunofluorescence and flow cytometry analysis: correlation of Leu-2, Leu-3, Leu-7, Leu-8, and Leu-11 cell surface antigen expression. J Immunol. 1984; 132:151. (Biology: Flow cytometry).
      23. Maddon PJ, Dalgleish AG, McDougal JS, Clapham PR, Weiss RA, Axel R. The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain.. Cell. 1986; 47(3):333-48. (Biology: Flow cytometry). View Reference
      24. Michie SA, Garcia CF, Strickler JG, Dailey MO, Rouse RV, Warnke RA. Expression of the Leu-8 antigen by B-cell lymphomas. Am J Clin Pathol. 1987; 88(4):486-490. (Biology: Flow cytometry). View Reference
      25. Morimoto C, Letvin NL, Distaso JA, Aldrich WR, Schlossman SF. The isolation and characterization of the human suppressor/inducer T-cell subset. J Immunol. 1985; 134:1508-1515. (Biology: Flow cytometry).
      26. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology: Flow cytometry). View Reference
      27. Roederer M, Bigos M, Nozaki T, Stovel RT, Parks DR, Herzenberg LA. Heterogeneous calcium flux in peripheral T cell subsets revealed by five-color flow cytometry using log-ratio circuitry.. Cytometry. 1995; 21(2):187-96. (Biology: Flow cytometry). View Reference
      28. Roederer M, Dubs JG, Anderson MT, Raju PA, Herzenberg LA, Herzenberg LA. CD8 naive T cell counts decrease progressively in HIV-infected adults. J Clin Invest. 1995; 95:2061-2066. (Biology: Flow cytometry).
      29. Rose LM, Ginsberg AH, Rothstein TL, Ledbetter JA, Clark EA. Selective loss of a subset of T helper cells in active multiple sclerosis.. Proc Natl Acad Sci USA. 1985; 82(21):7389-93. (Biology: Flow cytometry). View Reference
      30. Rudd CE, Burgess KE, Barber EK, Schlossman SF. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.
      31. Serra HM, Krowka JF, Ledbetter JA, Pilarski LM. Loss of CD45R (Lp220) represents a post-thymic T cell differentiation event.. J Immunol. 1988; 140(5):1435-41. (Biology: Flow cytometry). View Reference
      32. Sobel RA, Hafler DA, Castro EE, Morimoto C, Weiner HL. The 2H4 (CD45R) antigen is selectively decreased in multiple sclerosis lesions.. J Immunol. 1988; 140(7):2210-4. (Biology: Flow cytometry). View Reference
      33. Watanabe N, De Rosa SC, Cmelak A, et al. Long-term depletion of naive T cells in patients treated for Hodgkin's disease. Blood. 1997; 90:3662-3672. (Biology: Flow cytometry).
      34. Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983; 131(1):212-216. (Biology: Flow cytometry). View Reference
      35. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology: Flow cytometry).
      View All (35) View Less
      340977 Rev. 2

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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