Built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis,1* the innovative BD OneFlow™ Solution brings the standardization of leukemia and lymphoma immunophenotyping one step forward, improving laboratory efficiency and enabling reliability and accuracy of results for clinical decisions and ultimately patient outcome.2, 3 The BD OneFlow™ Solution is a comprehensive set of reagents (LST, B-CLPD T1, PCST, PCD and ALOT), setup beads, protocols, and assay acquisition and analysis templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations.
Learn more from the BD OneFlow™ Solution brochure.
BD OneFlow™ Lymphoid Screening Tube (LST)
The BD OneFlow™ LST is a preconfigured, ready-to-use 8-color 12-antibodies reagent that is provided in a single-test tube format. It is intended for flow cytometric immunophenotyping of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages in peripheral blood, bone marrow and lymph nodes as an aid in diagnosis of hematological disorders.
BD OneFlow™ B-cell Chronic Lymphoproliferative Diseases Tube 1 (B-CLPD T1)
The BD OneFlow™ B-CLPD T1 is a preconfigured, ready-to-use 8-color reagent provided in a single-test tube format. It shall be used for specimens with B cell lineage populations needing further investigation in combination with the BD OneFlow™ LST. B-CLPD T1 is intended for flow cytometric immunophenotyping of B cells in peripheral blood and bone marrow as an aid in the diagnosis of chronic lymphocytic leukemia (CLL) and other B cell chronic lymphoproliferative diseases.
BD OneFlow™ Plasma Cell Screening Tube (PCST)
The BD OneFlow™ PCST is a preconfigured, ready-to-use 8-color reagent comprising two tubes—one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube). It is intended for flow cytometric immunophenotyping of normal polyclonal and aberrant plasma cell populations in bone marrow as an aid in the diagnosis of hematological disorders.
BD OneFlow™ Plasma Cell Disorders (PCD) Tube
The BD OneFlow™ PCD Tube is a preconfigured, ready-to-use 8-color reagent provided in a single-test tube format. When run in parallel with the BD OneFlow™ PCST, it is intended for flow cytometric immunophenotyping of normal and aberrant plasma cells in bone marrow as an aid in the diagnosis of multiple myeloma or other plasma cell disorders.
BD OneFlow™ Acute Leukemia Orientation Tube (ALOT)
The BD OneFlow™ ALOT is a preconfigured, ready-to-use 8-color reagent comprising two tubes—one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube). The tube is intended for flow cytometric immunophenotyping of aberrant immature populations of hematopoietic cells (lymphoid and non-lymphoid lineage) in bone marrow and peripheral blood as an aid in the diagnosis of acute lymphoblastic leukemia and non-lymphoid acute leukemia.
Why BD OneFlow™ Solution?
- Efficient workflows to reduce manual workload
- Ready-to-use, single-test tube format for direct specimen staining
- Convenient dried format
- Comprehensive solution of reagents, beads, protocols and assay templates
Our Leadership
Our innovative BD OneFlow™ Solution brings the standardization of leukemia and lymphoma immunophenotyping one step forward.
View videos describing how standardization, efficiency, accuracy and reproducibility can be achieved using the BD OneFlow™ Solution on the BD FACSCanto™ II Flow Cytometer.
References
- van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120
- Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779
- Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122
