Preparation And Storage
Recommended Assay Procedures
For a working solution, dilute to 10 mM in sterile deionized water. [The molar mass of Y-27632 is 338.3 g/mol.]
Small aliquots may be stored at -20°C, avoiding repeated freeze-thaw cycles.
Use at 10 µM in culture medium. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Avoid contact with skin and eyes.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- mTESR™1 is a trademark of StemCell Technologies.
- Accutase is a registered trademark of Innovative Cell Technologies, Inc.
Companion Products
The ROCK inhibitor, Y-27632, is a selective inhibitor of the p160-Rho-associated coiled kinase (ROCK) and is a factor that enhances embryonic stem cell survival upon single cell dissociation. In addition to increasing cell recovery after dissociation, Y-27632 has been used for various other applications in stem cell research including cryopreservation, sorting, reprogramming, transplantation, and differentiation.
Development References (5)
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Braam SR, Nauw R, Ward-van Oostwaard D, et al. Inhibition of ROCK improves survival of human embryonic stem cell-derived cardiomyocytes after dissociation. Ann N Y Acad Sci. 2010; 1188:52-57. (Methodology: Cell culture). View Reference
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Emre N, Vidal JG, Elia J, et al. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. PLoS ONE. 2010; 5(8):e12148. (Methodology: Cell culture). View Reference
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Li X, Meng G, Krawetz R, Liu S, et al. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation. Stem Cells Dev. 2008; 17(6):1079-1085. (Methodology: Cell culture). View Reference
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Narumiya S, Ishizaki T, Uehata M. Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol. 2000; 325:273-284. (Biology: Cell culture). View Reference
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Watanabe K, Ueno M, Kamiya D. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol. 2007; 25(6):681-686. (Methodology: Cell culture). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
